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324727 Endoglycosidase F3, Elizabethkingia meningosepticum, Recombinant, E. coli

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324727
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Overview

Products

Catalogue NumberPackaging Qty/Pack
324727-200MIU Plastic ampoule 200 miu
Description
OverviewRecombinant, Elizabethkingia meningosepticum endoglycosidase F3 expressed in E. coli. Cleaves asparagine-linked or free biantennary and triantennary complex, and Man3GlcNAc oligosaccharides from glycoproteins. This enzyme cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide, generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine residue. Core fucosylation increases the activity of Endo F3 up to 40 fold. Exhibits no activity on high mannose and hybrid molecules. Less sensitive to protein conformation than N-Glycosidase F (Cat. No. 362185) and therefore is more suitable for deglycosylation of native proteins.
Note: 1 mU = 1 milliunit.
Catalogue Number324727
Brand Family Calbiochem®
SynonymsEndo-β-N-acetylglucosaminidase F3, Endo F3
References
ReferencesTarentino, A.L., et al. 1995. Glycobiology 5, 599.
Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.
Tarentino, A.L., et al. 1993. J. Biol. Chem. 268, 9702.
Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.
Product Information
Activity≥5 units/ml
Unit of DefinitionOne unit is defined as the amount of enzyme that will release N-linked oligosaccharides from 1.0 µmol porcine fibrinogen per min at 37°C, pH 5.5.
EC number3.2.1.96
FormLiquid
FormulationIn 20 mM Tris-HCl, pH 7.5.
Quality LevelMQ100
Biological Information
Specific Activity≥30 units/mg protein
Physicochemical Information
ContaminantsProteases: none detected
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Global Trade Item Number
Catalogue Number GTIN
324727-200MIU 04055977216011