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239835 Cyclosporin A, Tolypocladium inflatum - CAS 59865-13-3 - Calbiochem

Cyclosporin A, CAS 59865-13-3, is an immunosuppressant that induces apoptosis in rat thymocytes and in the murine B cell lymphoma cell line, WEH1-231. Induces cardiomyocytes formation from ESCs.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Primary Target: NOS induced by interlukin-1-α
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
59865-13-3C₆₂H₁₁₁N₁₁O₁₂
Description
OverviewCyclic oligopeptide with immunosuppressant properties. Induces apoptosis in rat thymocytes and in the murine B cell lymphoma cell line, WEH1-231. However, prevents anti-IgM and ionomycin-induced apoptosis in BLB cell lines. The complex of cyclosporin A with cyclophilin inhibits protein phosphatase 2B with nanomolar affinity. Inhibits nitric oxide synthesis induced by interleukin-1α, lipopolysaccharides, and TNF-α. Induces cardiomyocytes from embryonic stem cells.
Catalogue Number239835
Brand Family Calbiochem®
References
ReferencesYan, P., et al. 2008. Biochem. Biophys. Res. Commun. in press.
Hattori, Y., and Naranishi, N. 1995. Cell Immunol. 165, 7.
Marumo, T., et al. 1995. Hypertension 25, 764.
Crabtree, G.R., and Clipstone, N.A. 1994. Annu. Rev. Biochem. 63, 1045.
Gottschalk, A.R., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 7350.
Montague, J.W., et al. 1994. J. Biol. Chem. 269, 18877.
Fast, D.J., et al. 1993. J. Interferon Res. 13, 235.
Liu, J. 1993. Trends Pharmacol. Sci. 14, 182.
Fruman, D.A., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 3686.
Product Information
CAS number59865-13-3
ATP CompetitiveN
FormWhite to off-white solid
Hill FormulaC₆₂H₁₁₁N₁₁O₁₂
Chemical formulaC₆₂H₁₁₁N₁₁O₁₂
ReversibleN
Structure formula ImageStructure formula Image
Quality LevelMQ200
Applications
Biological Information
Primary TargetNOS induced by interlukin-1-α
Purity≥97% (dry basis)
Physicochemical Information
Cell permeableN
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSGZ4120000
Safety Information
R PhraseR: 40

Limited evidence of a carcinogenic effect.
S PhraseS: 22-24/25-36/37

Do not breathe dust.
Avoid contact with skin and eyes.
Wear suitable protective clothing and gloves.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Carcinogenic / Teratogenic
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
239835 0

Documentation

Cyclosporin A, Tolypocladium inflatum - CAS 59865-13-3 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Cyclosporin A, Tolypocladium inflatum - CAS 59865-13-3 - Calbiochem Certificates of Analysis

TitleLot Number
239835

References

Reference overview
Yan, P., et al. 2008. Biochem. Biophys. Res. Commun. in press.
Hattori, Y., and Naranishi, N. 1995. Cell Immunol. 165, 7.
Marumo, T., et al. 1995. Hypertension 25, 764.
Crabtree, G.R., and Clipstone, N.A. 1994. Annu. Rev. Biochem. 63, 1045.
Gottschalk, A.R., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 7350.
Montague, J.W., et al. 1994. J. Biol. Chem. 269, 18877.
Fast, D.J., et al. 1993. J. Interferon Res. 13, 235.
Liu, J. 1993. Trends Pharmacol. Sci. 14, 182.
Fruman, D.A., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 3686.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Protein Phosphatases Technical Bulletin

Citations

Title
  • Justin C. Mason, et al. (2004) Decay-accelerating actor induction on vascular endothelium by vascular endothelial growth factor (VEGF) is mediated via a VEGF receptor-2 (VEGF-R2)- and protein kinase C-α/ε (PKCα/ε)-dependent cytoprotective signaling pathway a. Journal of Biological Chemistry 279, 41611-41618.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision22-January-2009 JSW
    DescriptionMaterial is a nonpolar cyclic oligopeptide with immunosuppressant activity. Appears to act via interaction with the intracellular protein cyclophilin. The cyclophilin-cyclosporin A complex is a potent inhibitor of the protein phosphatase activity of calcineurin (protein phosphatase 2B, PP2B). Induces apoptosis in rat thymocytes and in the murine B-cell lymphoma cell line, WEHI-231; however, it prevents anti-IgM and ionomycin-induced apoptosis in BLB cell lines. Inhibits nitric oxide synthesis induced by interleukin 1α, lipopolysaccharides, and TNFα. Induces cardiomyocytes from embryonic stem cells.
    FormWhite to off-white solid
    CAS number59865-13-3
    RTECSGZ4120000
    Chemical formulaC₆₂H₁₁₁N₁₁O₁₂
    Structure formulaStructure formula
    Purity≥97% (dry basis)
    SolubilityDMSO (25 mg/ml), Ethanol, Methanol, or chloroform
    Storage +2°C to +8°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
    Toxicity Carcinogenic / Teratogenic
    Merck USA index14, 2752
    ReferencesYan, P., et al. 2008. Biochem. Biophys. Res. Commun. in press.
    Hattori, Y., and Naranishi, N. 1995. Cell Immunol. 165, 7.
    Marumo, T., et al. 1995. Hypertension 25, 764.
    Crabtree, G.R., and Clipstone, N.A. 1994. Annu. Rev. Biochem. 63, 1045.
    Gottschalk, A.R., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 7350.
    Montague, J.W., et al. 1994. J. Biol. Chem. 269, 18877.
    Fast, D.J., et al. 1993. J. Interferon Res. 13, 235.
    Liu, J. 1993. Trends Pharmacol. Sci. 14, 182.
    Fruman, D.A., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 3686.
    Citation
  • Justin C. Mason, et al. (2004) Decay-accelerating actor induction on vascular endothelium by vascular endothelial growth factor (VEGF) is mediated via a VEGF receptor-2 (VEGF-R2)- and protein kinase C-α/ε (PKCα/ε)-dependent cytoprotective signaling pathway a. Journal of Biological Chemistry 279, 41611-41618.
    		•