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S8000 CpGenome™ Universal Methylated Mouse DNA Standard Set

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S8000
5 µg   
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Overview

Replacement Information
Description
Catalogue NumberS8000
Brand Family Upstate
Trade Name
  • CpGenome
  • Upstate
DescriptionCpGenome™ Universal Methylated Mouse DNA Standard Set
OverviewThe CpGenome™ Universal Methylated Mouse DNA Standard contains universally methylated mouse genomic DNA isolated from male Balb/c mice and enzymatically methylated at all CpGs by M.SssI methyltransferase. It can be used as a positive control for gene methylation studies, and is fully compatible with the CpGenome™ Turbo Bisulfite kit (Cat. No. S7847) and CpG WIZ® methylation-specific amplification systems.

Materials Provided:

One vial containing 5 µg (20 µl) of CpGenome™ Universal Methylated Mouse DNA Standard at a concentration of 250 ng/µL.

Validation:

Methylation-specific PCR (MSP) was performed on the CpGenome™ Universal Methylated Mouse DNA after bisulfite modification with the CpGenome™ Turbo Bisulfite kit (Cat. No. S7847). Three sets of primers from the CpG WIZ® Oct-4 kits (Catalog No. S7840) were used in this assay: the U primer set, which anneals to unmethylated bisulfite-modified DNA; M primers, which anneal to methylated bisulfite-modified sequences; and W primers, which anneal to unmethylated or methylated DNA that has not undergone bisulfite modification. Only the M primers generated product for this Standard.

CpGenome and CpG WIZ are trademarks of Serologicals Corporation. CpG WIZ Methylation Products apply to technologies exclusively licensed from The Johns Hopkins University School of Medicine. Methylation-specific PCR (MSP) technology is covered under U.S. Patent # 5,786,146.
References
Product Information
PresentationLiquid in buffer containing 10 mM Tris-HCl, 1 mM EDTA, pH 8.0
Quality LevelMQ100
Applications
ApplicationThe CpGenome Universal Methylated Mouse DNA Standard contains universally methylated mouse genomic DNA isolated from male Balb/c mice & enzymatically methylated at all CpGs by M.SssI methyltransferase.
Application NotesFor MSP primer design, please use the MethPrime software package. Click here
Biological Information
Species Reactivity
  • Mouse
Modifications
  • Methylation
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended Storage: Stable at -20°C for up to 6 months from date of receipt.
Packaging Information
Material Size5 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
S8000 04053252648397

Documentation

CpGenome™ Universal Methylated Mouse DNA Standard Set SDS

Title

Safety Data Sheet (SDS) 

CpGenome™ Universal Methylated Mouse DNA Standard Set Certificates of Analysis

TitleLot Number
CpGenome Universal Methylated Mouse DNA Standard - Q2023990 Q2023990
CpGenome™ Universal Methylated -2822806 2822806
CpGenome™ Universal Methylated Mouse DNA Standard 2937870
CpGenome™ Universal Methylated Mouse DNA Standard 3137734
CpGenome™ Universal Methylated Mouse DNA Standard 3082562
CpGenome™ Universal Methylated Mouse DNA Standard - 3305936 3305936
CpGenome™ Universal Methylated Mouse DNA Standard - 3430351 3430351
CpGenome™ Universal Methylated Mouse DNA Standard - 3520180 3520180
CpGenome™ Universal Methylated Mouse DNA Standard - 3709642 3709642
CpGenome™ Universal Methylated Mouse DNA Standard - 3757117 3757117

Brochure

Title
Product Selection Guide - Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.
Shaping Epigenetics Discovery - DNA Methylation Brochure

Technical Info

Title
White Paper - The Message in the Marks: Deciphering Cancer Epigenetics