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70526 Clonables™ Ligation/Transformation Kit - Novagen

Simple, reproducible ligation and transformation—in as little as 23 minutes

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
70526
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Overview

Products

Catalogue NumberPackaging Qty/Pack
70526-3 Plastic ampoule 11 rxn
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Description
OverviewThe Clonables™ Kit enables convenient, dependable, high-efficiency ligation and transformation of any compatible DNA ends. The kit features a unique, universal Ligation Premix, containing ligase, buffer, and cofactors, which supports ligation of any type of DNA cohesive or blunt ends in a 15-minute reaction. Ligated DNA is transformed into NovaBlue Singles™ Competent Cells, which use a streamlined protocol that takes less than 8 minutes for ampicillin-resistant plasmids and 38 minutes for other antibiotic-resistant plasmids. This kit can be used with a variety of cloning vectors and is compatible with any type of DNA end, without altering the ends or desired cloning junctions.

The Clonables Kit contains sufficient reagents to perform 11 ligation and transformation reactions, and includes a control vector and insert mix to verify performance.
Catalogue Number70526
Brand Family Novagen®
Features and benefits
  • Fast! 15-minute ligation, 8-minute transformation with ampicillin selection
  • Ligation components premixed to decrease pipetting steps and increase reliability
  • One reaction, optimized for cohesive ends, single base overhangs, and blunt ends
  • Single-use competent cells eliminate need to aliquot, freeze/thaw, or waste partially used vials
  • Compatible with PCR buffer, TE, restriction enzyme buffer, or End Conversion Mix
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    Product Information
    Components
    Quality LevelMQ100
    Storage and Shipping Information
    Ship Code Dry Ice Only
    Toxicity Multiple Toxicity Values, refer to MSDS
    Storage ≤ -70°C
    Avoid freeze/thaw Avoid freeze/thaw
    Do not freeze Ok to freeze
    Global Trade Item Number
    Catalogue Number GTIN
    70526-3 04055977273595