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218807 Caspase-9, Human, Recombinant, E. coli

Caspase-9, Human, Recombinant is expressed in E. coli as a single polypeptide. Fused at the N- and C- termini to a His•Tag® sequence. Undergoes spontaneous autoprocessing to yield the active enzyme.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: ICE-LAP6, Mch6

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Overview

Replacement Information
Description
OverviewRecombinant, human caspase-9 fused at the N- and C- termini to a His•Tag® sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. MW: 11000 and 20000.
Catalogue Number218807
Brand Family Calbiochem®
SynonymsICE-LAP6, Mch6
References
ReferencesQin, H., et al. 1999. Nature 399, 549.
Li, P., et al. 1997. Cell 91, 479.
Salvesen, G.S., et al. 1997. Cell 91, 443.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will cleave 1.0 nmol substrate Ac-LEHD-<i>p</i>NA (<a href ="/Products/ProductDisplay.asp→catNO=218805">Cat. No. 218805</a>) per h at 37°C, pH 7.2.
FormLyophilized
FormulationLyophilized from 130 mM NaCl, 20 mM Tris, 4 mM (NH₄)₂SO₄
Quality LevelMQ100
Applications
Biological Information
Purity≥70% by SDS-PAGE
Specific Activity≥400 units/mg protein
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage ≤ -70°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 4 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
218807 0

Documentation

Caspase-9, Human, Recombinant, E. coli SDS

Title

Safety Data Sheet (SDS) 

Caspase-9, Human, Recombinant, E. coli Certificates of Analysis

TitleLot Number
218807

References

Reference overview
Qin, H., et al. 1999. Nature 399, 549.
Li, P., et al. 1997. Cell 91, 479.
Salvesen, G.S., et al. 1997. Cell 91, 443.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision04-June-2008 RFH
SynonymsICE-LAP6, Mch6
DescriptionRecombinant, human caspase-9 fused at the N- and C- termini to a His•Tag® sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. MW: 11000 and 20000.
FormLyophilized
FormulationLyophilized from 130 mM NaCl, 20 mM Tris, 4 mM (NH₄)₂SO₄
Recommended reaction conditions50 mM HEPES, 50 mM NaCl, 10 mM DTT, 10 mM EDTA, 5% glycerol, 0.1% CHAPS, pH 7.2. Recommended use: 1 unit per assay.
Purity≥70% by SDS-PAGE
Specific activity≥400 units/mg protein
Unit definitionOne unit is defined as the amount of enzyme that will cleave 1.0 nmol substrate Ac-LEHD-pNA (Cat. No. 218805) per h at 37°C, pH 7.2.
SolubilityPBS containing 15% glycerol (1 U/µl)
Storage ≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 4 months at -20°C.
Toxicity Standard Handling
ReferencesQin, H., et al. 1999. Nature 399, 549.
Li, P., et al. 1997. Cell 91, 479.
Salvesen, G.S., et al. 1997. Cell 91, 443.