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218828 Caspase-1 Substrate XV, Cell-Permeable, Fluorogenic

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Nα-5-Tetramethylrhodaminyl-YVADAC(S-acrylodan)-OH, TMR-YVADAC(AD)

218828
  
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Overview

Replacement Information
Description
Overview

This product has been discontinued.



Please refer to our complete listing of caspase substrates for possible alternatives.






A cell-permeable, internally-quenched fluorogenic peptide substrate that is useful for detecting in vivo caspase-1-like protease activity in living cells or tissues. Efficiently cleaved by caspase-1 in vitro at the peptide bond between Asp and Ala residues (kcat/Km ~ 8.5 x 10-2 µM-1s-1) with an increase in fluorescence intensity (~525 nm) when excited at 380 nm. Both time-dependent and sub-cellular localization of caspase-1-like activity could be visualized in glutamate-induced rat hippocampal cell death.
Catalogue Number218828
Brand Family Calbiochem®
SynonymsNα-5-Tetramethylrhodaminyl-YVADAC(S-acrylodan)-OH, TMR-YVADAC(AD)
References
ReferencesNishii, W., et al. 2002. FEBS Lett. 518, 149.
Product Information
FormRed-purple lyophilized solid
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₆₇H₇₅N₉O₁₅S
Chemical formulaC₆₇H₇₅N₉O₁₅S
Applications
Biological Information
Purity≥90% by HPLC
Physicochemical Information
Peptide SequenceTAMRA(5)-Tyr-Val-Ala-Asp-Ala-Cys(S-acrylodan)-OH
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Protect from Moisture Protect from moisture
Do not freeze Ok to freeze
Special InstructionsUnstable in solution; reconstitute just prior to use.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
218828 0

Documentation

Caspase-1 Substrate XV, Cell-Permeable, Fluorogenic Certificates of Analysis

TitleLot Number
218828

References

Reference overview
Nishii, W., et al. 2002. FEBS Lett. 518, 149.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-June-2008 RFH
SynonymsNα-5-Tetramethylrhodaminyl-YVADAC(S-acrylodan)-OH, TMR-YVADAC(AD)
DescriptionA cell-permeable, internally-quenched fluorogenic peptide substrate that is useful for detecting caspase-1-like protease activity in living cells or cultured tissues. Efficiently cleaved by caspase-1 in vitro at the peptide bond between Asp and Ala residues (kcat/Km ~8.5 x 10-2µM-1s-1) with an increase in fluorescence intensity at ~520 nm. Excitation max: ~380 nm; emission max: ~520 nm.
FormRed-purple lyophilized solid
FormulationSupplied as a trifluoroacetate salt.
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₆₇H₇₅N₉O₁₅S
Peptide SequenceTAMRA(5)-Tyr-Val-Ala-Asp-Ala-Cys(S-acrylodan)-OH
Purity≥90% by HPLC
SolubilityDMSO (5 mg/ml)
Storage Protect from moisture
Protect from light
-20°C
Do Not Freeze Ok to freeze
Special InstructionsUnstable in solution; reconstitute just prior to use.
Toxicity Standard Handling
ReferencesNishii, W., et al. 2002. FEBS Lett. 518, 149.