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218709 Casein Kinase II, α2, Active, Human, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: CK2α2, CSNK2A2

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Overview

Replacement Information
Description
Overview

This product has been discontinued.



Recombinant, human casein kinase II, α2, (CKII, α2) fused to maltose binding protein (MBP) at the N-terminus and expressed in E. coli. CKII, α2 is a catalytic subunit of the serine/threonine protein kinase CKII. It is suitable for use in reactions where autophosphorylation of the regulatory CKII β-subunit is not desired. Shown to be extremely salt-sensitive with respect to its activity.
Catalogue Number218709
Brand Family Calbiochem®
SynonymsCK2α2, CSNK2A2
Application Data
Increasing concentrations of Casein Kinase II, α2 were assayed according to the protocol outlined above.
References
ReferencesAntonelli, M., et al. 1997. Eur. J. Biochem. 241, 272.
Xu, X., et al. 1997. Genomics 48, 79.
Boldyreff, B., et al. 1994. Cell. Mol. Biol. Res. 40, 391.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme required to transfer 1 pmol phosphate to RRRADDSDDDDD peptide substrate per min at 30°C.
FormLiquid
FormulationIn 50 mM Tris-HCl, 500 mM NaCl, 10 mM DTT, pH 8.5.
Quality LevelMQ100
Applications
Biological Information
Purity≥ 90% by SDS PAGE
Specific Activity≥841.819 Units/mg protein
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
218709 0

Documentation

Casein Kinase II, α2, Active, Human, Recombinant, E. coli SDS

Title

Safety Data Sheet (SDS) 

Casein Kinase II, α2, Active, Human, Recombinant, E. coli Certificates of Analysis

TitleLot Number
218709

References

Reference overview
Antonelli, M., et al. 1997. Eur. J. Biochem. 241, 272.
Xu, X., et al. 1997. Genomics 48, 79.
Boldyreff, B., et al. 1994. Cell. Mol. Biol. Res. 40, 391.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision17-April-2017 JSW
SynonymsCK2α2, CSNK2A2
Application Data
Increasing concentrations of Casein Kinase II, α2 were assayed according to the protocol outlined above.
DescriptionRecombinant, human casein kinase II, α2, (CKII, α2) fused to maltose binding protein (MBP) at the N-terminus and expressed in E. coli. CKII, α2 is a catalytic subunit of the serine/threonine protein kinase CKII. It is suitable for use in reactions where autophosphorylation of the regulatory CKII β-subunit is not desired. Shown to be extremely salt-sensitive with respect to its activity.
FormLiquid
FormulationIn 50 mM Tris-HCl, 500 mM NaCl, 10 mM DTT, pH 8.5.
Concentration Label Please refer to vial label for lot-specific concentration
Recommended reaction conditions
Kinase Activity Assay Required Materials •Assay Buffer: 25 mM β-glycerophosphate, 20 mM Tris-HCl, 5 mM EGTA, 1 mM DTT, pH 7.5 •Substrate: 800 µM RRRDDDSDDD peptide substrate •CKII, α2: 5-10 ng/µl CKII, α2 diluted in Assay Buffer just prior to use •Magnesium/ATP Mix: 75 mM MgCl2, 500 µM ATP •Diluted [γ-32P]ATP/Magnesium/ATP Mix: Mix 197 µl Magnesium/ATP Mix with 3 µl (30 µCi) [γ-32P]ATP (3000 Ci/mmol) Protocol 1. Pipette 10 µl Assay Buffer into a clean microcentrifuge tube on ice. 2. Add 10 µl Substrate. 3. Add 10 µl diluted CKII, α2. 4. Add 10 µl Diluted [γ-32P]ATP/Magnesium/ATP Mix. 5. Incubate at 37° for 10 min. 6. Stop the reaction by placing the tubes on ice. 7. Spot 10 µl of each reaction on P81 paper and allow to bind for 30 s. 8. Wash by immersing the paper in 0.75% phosphoric acid and gently shaking. Repeat for a total of 3 washes. 9. Wash as in step 8 using acetone. 10. Dry using an infrared light. 11. Determine the cpm using a scintillation counter or imager.
Purity≥ 90% by SDS PAGE
Specific activity≥841.819 Units/mg protein
Unit definitionOne unit is defined as the amount of enzyme required to transfer 1 pmol phosphate to RRRADDSDDDDD peptide substrate per min at 30°C.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesAntonelli, M., et al. 1997. Eur. J. Biochem. 241, 272.
Xu, X., et al. 1997. Genomics 48, 79.
Boldyreff, B., et al. 1994. Cell. Mol. Biol. Res. 40, 391.