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CYT294 Sigma-AldrichC-Reactive Protein ELISA Kit, Rat

The rat C-Reactive Protein (CRP) kit is a double polyclonal antibody sandwich enzyme immunoassay (EIA), which measures rat CRP.

C-Reactive Protein ELISA Kit, Rat MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
References
User Guides

Key Applications: ELISA Research Category: Metabolism, Cell Signaling, Epigenetics & Nuclear Function

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Overview

Replacement Information

Key Spec Table

Analytes Available	Species Reactivity	Key Applications
C-Reactive Protein	R	ELISA

Description
Catalogue Number	CYT294
Brand Family	Chemicon®
Trade Name	Chemicon
Description	C-Reactive Protein ELISA Kit, Rat
Overview	Introduction The rat C-Reactive Protein (CRP) kit is a double polyclonal antibody sandwich enzyme immunoassay (EIA), which measures rat CRP. Test Principle With the rat CRP ELISA, standards, quality controls and samples of sera are incubated in microtitration wells coated with polyclonal anti-rat CRP antibody. After a thorough wash, polyclonal anti-rat CRP antibody labelled with horseradish peroxidase (HRP) is added to the wells and incubated with the immobilized antibody-CRP complex. Following another washing step, the remaining HRP-conjugated antibody is allowed to react with the substrate and tetramethylbenzidine. The reaction is stopped by addition of acidic solution, and absorbance of the resulting yellow color product is measured spectrophotometrically at 450 nm. The absorbance is proportional to the concentration of CRP. A standard curve is constructed by plotting absorbance values versus CRP concentrations of standards, and concentrations of unknown samples are determined using this standard curve.
Alternate Names	CRP
Materials Required but Not Delivered	1. Test tubes for diluting samples 2. Precision pipettes to deliver 2--1000 mL 3. Microplate reader with 450 ± 10 nm filter 4. Software package facilitating data generation and analysis 5. Microtitration plate washer (optional) [Manual washing is possible but not preferable.] 6. Absorbent material for blotting the microwell plate 7. Clean 250-500 mL wash bottle for buffer 8. Adhesive cover for microplates 9. Deionized (distilled) water

References

Product Information
Components	Microwell Strips (96 wells), coated with Anti-rat CRP Antibody, vacuum sealed (Catalog No. CYT294a) Conjugate Concentrate (100x) (Anti-rat CRP Antibody, Horseradish Peroxidase Conjugate), 0.12 mL (Catalog No. CYT294b) Rat CRP Standard, 1.33 mg/mL (10x), 0.25 mL (Catalog No. CYT294c) Substrate (TMB) Solution, 12 mL, Protect from light. Non-carcinogenic. (Catalog No. CYT294d) Stop Solution (diluted phosphoric acid), 12 mL (Catalog No. CYT294e) Wash Buffer, 1 packet (PBS with 0.05% Tween 20, pH 7.4 when reconstituted to 1 liter of distilled water) (Catalog Number CYT294f)
HS Code	3002 15 90
Quality Level	MQ100

Applications
Application	The rat C-Reactive Protein (CRP) kit is a double polyclonal antibody sandwich enzyme immunoassay (EIA), which measures rat CRP.
Key Applications	ELISA
Application Notes	The rat C-reactive protein (CRP) kit is designed to measure the amount of rat CRP in serum or plasma. There are enough reagents included in this kit for one 96-well immuno-assay plate. We recommend running duplicate wells for samples and standards. For Research Use Only; Not for use in diagnostic procedures.

Biological Information
Species Reactivity	Rat
Analytes Available	C-Reactive Protein
UniProt Number	P14847

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	For research use only. Not for use in diagnostic procedures.

Storage and Shipping Information
Storage Conditions	Store the kit at 2° to 8°C for up to 6 months after date of receipt or until expiration date. Precautions · Avoid contact with the acidic Stop Solution and Substrate Solution, which contains urea peroxide. Wear gloves and eye protection when handling these reagents. In case of contact with the Stop Solution and the Substrate Solution wash skin thoroughly with water and seek medical attention, when necessary. · HRP-labeled conjugate and TMB-substrate are photosensitive and are packaged in a protective opaque bottle. Store in the dark and return to storage after use. · Wear gloves and laboratory coats when handling the kit. · The materials must not be pipetted by mouth. · Do not drink, eat or smoke in the areas where the kit is being handled. · Reagents with different lot numbers should not be mixed.

Storage and Shipping Information

Storage Conditions

Store the kit at 2° to 8°C for up to 6 months after date of receipt or until expiration date.

Precautions

· Avoid contact with the acidic Stop Solution and Substrate Solution, which contains urea peroxide. Wear gloves and eye protection when handling these reagents. In case of contact with the Stop Solution and the Substrate Solution wash skin thoroughly with water and seek medical attention, when necessary.

· HRP-labeled conjugate and TMB-substrate are photosensitive and are packaged in a protective opaque bottle. Store in the dark and return to storage after use.

· Wear gloves and laboratory coats when handling the kit.

· The materials must not be pipetted by mouth.

· Do not drink, eat or smoke in the areas where the kit is being handled.

· Reagents with different lot numbers should not be mixed.

Packaging Information
Material Size	1 plate
Material Package	96 wells

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
CYT294	04053252674648

Documentation

C-Reactive Protein ELISA Kit, Rat SDS

Title
Safety Data Sheet (SDS)

References

Reference overview	Pub Med ID
Relationship between osteoinductive characteristics of biocomposite material and physicochemical characteristics of coating. M Z Fedorova,S V Nadezhdin,Yu R Kolobov,M B Ivanov,N A Pavlov,E V Zubareva Bulletin of experimental biology and medicine 148 2009 Show Abstract The osteoinductive characteristics of hydroxylapatite-based coating with different physicochemical characteristics were studied. The formation of the bone tissue on the studied types of the implant coating differed by the pattern and rate of formation of structural elements of the bone. Clear-cut phase-wise osteogenesis was seen on combined calcium phosphate coating: intense formation of the fibrous interstitial substance (due to cellular structure of hydroxylapatite) and slow maturing of cell elements. Bilayer composite coating provided (during the same period) the formation of more mature connective tissue. This phenomenon can be explained by better bioavailability of the coating material. The properties of combined calcium phosphate coating can be evaluated as mainly osteoconductive, while those of bilayer composite coating as osteoinductive.	20396802

Reference overview

Pub Med ID

User Guides

Title
Rat C-Reactive Protein (CRP) ELISA Kit

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