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SCT280 BioTracker™ Far Red Live Cell Cycle Assay Solution

Far red fluorescence probe for analysis of cell cycle by flow cytometry.

SCT280
50 tests  
Purchase on Sigma-Aldrich

Overview

Description
Catalogue NumberSCT280
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DescriptionBioTracker™ Far Red Live Cell Cycle Assay Solution
OverviewPropidium iodide (PI) is often used for cell cycle analysis by flow cytometry. Compared to this analysis method, the BioTracker™ Far Red Live Cell Cycle Assay Solution does not require cell fixation or permeabilization, and is permeable in live cells.

Use 5 uL of probe solution in 500 uL PBS for each sample. Vial contains enough for 50 tests.

The far red probe uses the 633 laser, enabling multiplexing with shorter wavelength probes by freeing the 488 laser for probes that emit in the green range of the spectrum.

IMPORTANT APPLICATION NOTES:
*Wash cells thoroughly to remove trypsin. Residual trypsin can confound analysis of flow cytometry populations.

*Adjust gates after cells are stained with probe

SPECTRAL PROPERTIES

Excitation: 633 - 647 nm
Emission: 695 LP or 780/60 BP.
A filter set for APC-Cy7 is suitable.
Alternate Names
  • cell cycle probe
  • cell cycle dye
  • cell cycle indicator
  • live cell probe
Background InformationThe control system of the cell cycle is closely linked to cell proliferation. Cell division in which one cell produces two daughter cells occurs as part of the cell cycle. The cell cycle can be assessed by analyzing cells stained for DNA using flow cytometry, followed by analysis of the proportion of stained nuclei in cell cycle phase. The cell cycle is classified into two major phases: interphase and the mitotic (M) phase. Interphase is composed of G1 phase (preparation for DNA synthesis which occurs prior to cell division), S phase (DNA synthesis and replication), and G2 phase (a single cell with duplicated chromosomes). M phase involves mitosis and cytokinesis. Cell cycle progression is regulated by cyclins and cyclin-dependent kinases. To determine the action of anticancer drugs and others, it is important to analyze the mechanism of cell-cycle checkpoint.
Product Information
Detection methodFlow cytometry Fluorescence
FormatLiquid Reagent
PresentationLiquid reagent. Aqueous solution.
Quality LevelMQ100
Applications
ApplicationFar red fluorescence probe for analysis of cell cycle by flow cytometry.
Key Applications
  • Cell Based Assays
  • Fluorescence
  • Flow Cytometry
  • Fluorescence Activated Cell Sorting (FACS)
Application NotesLive cell probe for fluorescent cell cycle analysis. Unlike propidium iodide, this probe is membrane permeable and highly specific for DNA, which makes it possible to analyze the cell cycle by simply adding the reagent to a cell suspension.
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore BioTracker™ Far Red Live Cell Cycle Assay Solution at -20 °C, protected from light.
Packaging Information
Material Size50 tests
Global Trade Item Number
Catalogue Number GTIN
SCT280 04065269218586