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MABS2257-100UG Anti-sACDY10 Antibody, clone R21

Anti-sACDY10, clone R21, Cat. No. MABS2257, is a mouse monoclonal antibody that detects Adenylate cyclase type 10 (sAC) and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

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MABS2257-100UG
100 μg  
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Overview

Replacement Information
Description
Catalogue NumberMABS2257-100UG
DescriptionAnti-sACDY10 Antibody, clone R21
Alternate Names
  • Adenylate cyclase type 10
  • EC:4.6.1.1
  • AH-related protein
  • Adenylate cyclase homolog
  • Germ cell soluble adenylyl cyclase
  • hsAC
  • sAC
  • Testicular soluble adenylyl cyclase
Background InformationAdenylate cyclase type 10 (UniProt: Q96PN6; also known as EC:4.6.1.1, AH-related protein, Adenylate cyclase homolog, Germ cell soluble adenylyl cyclase, hsAC, sAC, Testicular soluble adenylyl cyclase) is encoded by the ADCY10 (also known as SAC) gene (Gene ID: 55811) in human. sAC catalyzes the formation of the signaling molecule cAMP. It is present in cytoplasm, nucleus, and mitochondria and is mainly detected in airway epithelial cells and testis. It is also expressed in several other tissues, but at much lower levels. It serves as a sensor to mediate responses to changes in cellular pH and bicarbonate levels and regulates pH-dependent V-ATPase recycling. sAC is shown to be activated by manganese or magnesium ions. In the presence of magnesium ions, it is activated by bicarbonate, however, in the presence of manganese ions its activity is inhibited by bicarbonate. In the absence of magnesium and bicarbonate, its activity is shown to be weakly activated by calcium ions. sAC plays a critical role in mammalian spermatogenesis by producing the cAMP, which regulates cAMP-responsive nuclear factors indispensable for sperm maturation in the epididymis. It induces capacitation, the maturational process that sperm undergo prior to fertilization and is also involved in ciliary beat regulation. sAC is shown to play a role in the pathogenesis of certain hyperproliferative skin disorders via modulation of gene expression. Its levels are shown to be upregulated in the nuclei of keratinocytes in certain hyperproliferative skin diseases, such as psoriasis and squamous cell carcinoma. However, it is shown that sAC is lost from the nucleus when a malignant epithelial tumor acquires invasive properties in the dermis. (Ref.: Zippin, JH., et al. (2010). J. Invest. Dermatol. 130(5); 1279-1287; Hess, KC., et al. (2005). Dev Cell. 9(2); 249-259; Pastor-Soler, N., et al. (2003). J. Biol. Chem. 278(49); 49523-49529).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-sACDY10, clone R21, Cat. No. MABS2257, is a mouse monoclonal antibody that detects Adenylate cyclase type 10 (sAC) and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesTested Applicaitons

Immunohistochemistry Applications: A representative lot detected sACDY10 in Immunohistochemistry applications (Pastor-Soler, N., et al. (2003). J Biol Chem.;278(49):49523-9).

Western Blotting Analysis: A representative lot detected sACDY10 in Western Blotting applications (Zippin, J.H., et al. (2003). FASEB J.;17(1):82-4; Pastor-Soler, N., et al. (2003). J Biol Chem.;278(49):49523-9; Hess, K.C., et al. (2005). Dev Cell.;9(2):249-59; Zippin, J.H., et al. (2010). J Invest Dermatol.;130(5):1279-87).

Immunocytochemistry Analysis: A representative lot detected sACDY10 in Immunocytochemistry applications (Zippin, J.H., et al. (2003). FASEB J.;17(1):82-4; Pastor-Soler, N., et al. (2003). J Biol Chem.;278(49):49523-9; Hess, K.C., et al. (2005). Dev Cell.;9(2):249-59; Zippin, J.H., et al. (2010). J Invest Dermatol.;130(5):1279-87).

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected sACDY10 in COS-7 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected sACDY10 in human melanoma tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenGST-tagged fusion protein corresponding to the 50 kDa splice variant of human soluble Adenylyl cyclase (sAC).
EpitopeN-terminal
CloneR21
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone R21 is a mouse monoclonal antibody that detects soluble adenylyl cyclase in multiple mammalian species.
IsotypeIgG1κ
Species Reactivity
  • Mouse
  • Canine
  • Monkey
  • Rat
  • Human
Species Reactivity NoteHuman, Canine, Monkey, Mouse, Rat.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ACDY10
Purification MethodProtein G purified
UniProt Number
Molecular Weight~75 kDa for the spice variant; 187.15 kDa calculated for full-length native protein. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting with GST-soluble adenylate cyclase fusion protein.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected GST-soluble Adenylate cyclase type 10 fusion protein.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS2257-100UG 04065266060041

Documentation

Anti-sACDY10 Antibody, clone R21 SDS

Title

Safety Data Sheet (SDS) 

Anti-sACDY10 Antibody, clone R21 Certificates of Analysis

TitleLot Number
Anti-sACDY10, clone R21 - 3852272 3852272
Anti-sACDY10, clone R21 - 3873615 3873615
Anti-sACDY10, clone R21 - 3888843 3888843
Anti-sACDY10, clone R21 - 3892247 3892247
Anti-sACDY10, clone R21 - 3896369 3896369
Anti-sACDY10, clone R21 - 3919629 3919629
Anti-sACDY10, clone R21 - 3939260 3939260
Anti-sACDY10, clone R21 - 3959208 3959208
Anti-sACDY10, clone R21 - 3959564 3959564
Anti-sACDY10, clone R21 - 3978943 3978943
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