Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

ABE2872 Anti-phospho-Smad2 (Ser465 Antibody, Ser467)

Anti-phospho-Smad2 (Ser465, Ser467), Cat. No. ABE2872, is a highly specific rabbit polyclonal antibody that targets Mothers against decapentaplegic homolog 2 (Smad2) and has been tested in Immunofluorescence, Immunoprecipitation, and Western Blotting.

Anti-phospho-Smad2 (Ser465 Antibody, Ser467) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: SMAD2, MADH2, MADR2 UniProt Number: Q15796
View Products on Sigmaaldrich.com
ABE2872
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIF, IP, WBRbUnpurifiedPolyclonal Antibody
Description
Catalogue NumberABE2872
DescriptionAnti-phospho-Smad2 (Ser465 Antibody, Ser467)
Alternate Names
  • Mothers against decapentaplegic homolog 2
  • MAD homolog 2
  • Mothers against DPP homolog 2
  • JV18-1
  • Mad-related protein 2
  • hMAD-2
  • SMAD family member 2
  • hSMAD2
Background InformationMothers against decapentaplegic homolog 2 (UniProt: Q15796; also known as MAD homolog 2, Mothers against DPP homolog 2, JV18-1, Mad-related protein 2, hMAD-2, SMAD family member 2, SMAD 2, Smad2, hSMAD2) is encoded by the SMAD2 (also known as MADH2, MADR2) gene (Gene ID: 4087) in human. Smad2 is a receptor-regulated Smad (R-Smad) that serves as an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor beta (TGF beta) and activin type 1 receptor kinases. It is expressed at high levels in skeletal muscle, endothelial cells, heart, and placenta. It contains one Mad homology (MH) 1 domain (aa 10-176), one MH2 domain (aa 274-467) and a PY motif (aa 221-225). Cytoplasmic Smad2 is directly phosphorylated on Ser465/Ser467 by TGF- receptor kinase I. These C-terminal phosphoserines of R-Smads are recognized by the MH2 domain of Smad4 that enables them to form a heterodimeric complex, which translocates to the nucleus where Smad proteins bind to their cognate DNA binding sites with low affinity. This binding is further enhanced in the presence of transcriptional co-activators. Acetylation Lys-19 by coactivators in response to TGF-beta signaling is shown to increase its transcriptional activity. Upon dephosphorylation by phosphatase PPM1A, Smad2 is released from the Smad2/Smad4 complex and is exported out of the nucleus.
References
Product Information
FormatUnpurified
PresentationRabbit polyclonal antiserum with 0.01% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-phospho-Smad2 (Ser465, Ser467), Cat. No. ABE2872, is a highly specific rabbit polyclonal antibody that targets Mothers against decapentaplegic homolog 2 (Smad2) and has been tested in Immunofluorescence, Immunoprecipitation, and Western Blotting.
Key Applications
  • Immunofluorescence
  • Immunoprecipitation
  • Western Blotting
Application NotesImmunofluorescence Analysis: A representative lot detected phospho-Smad2 (Ser465, Ser467) in prostate cancer tissue (Yakymovych, I., et. al. (2015). J Cell Biol. 210(2):319-32).

Western Blotting Analysis: A representative lot detected phospho-Smad2 (Ser465, Ser467) in TBP: PC-3U cells stimulated with 5 ng/ml TGF (Yakymovych, I., et. al. (2015). J Cell Biol. 210(2):319-32).

Immunoprecipitation Analysis: A representative lot detected phospho-Smad2 (Ser465, Ser467) in COS cells transfected with constitutively active versions of ALK5 (TLR-I)- and ALK6TL45-mediated phosphorylation (Persson, U., et. al. (1998). FEBS Lett. 434(1-2):83-7).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 4 amino acids surrounding pSerine from the C-terminus region of human Smad2.
EpitopeC-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects SMAD2 phosphorylated on Serine 465 and Serine 467.
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • SMAD2
  • MADH2
  • MADR2
Modifications
  • Phosphorylation
  • Phosphorylation
Purification MethodUnpurified
UniProt Number
Molecular Weight~56 kDa observed; 52.31 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEPG2 stimulated with TGFbeta1 cells.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected phospho-Smad2 (Ser465, Ser467) in HEPG2 stimulated with TGFbeta1 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE2872 04054839337123