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FCMAB108A4 Sigma-AldrichAnti-phospho-SMC1 (Ser957) Antibody, clone 5D11G5 Alexa Fluor® 488

This Anti-phospho-SMC1 (Ser957) Antibody, clone 5D11G5 Alexa Fluor 488 is validated for use in WB, FC for the detection of phospho-SMC1 (Ser957).

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Research Category: Epigenetics & Nuclear Function, Apoptosis & Cancer Research Sub-Category: Cell Cycle, DNA Replication & Repair Gene Symbol: SMC1A, SMCB, SMC1alpha, DXS423E, DKFZp686L19178, SMC1, SMC1L1, Smcb, OTTHUMP00000061876, KIAA0178, SB1.8, MGC138332, CDLS2, Sb1.8 UniProt Number: Q14683

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Key Spec Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, Xn, B	WB, FC	M	AlexaFluor®488	Monoclonal Antibody

Description
Catalogue Number	FCMAB108A4
Description	Anti-phospho-SMC1 (Ser957) Antibody, clone 5D11G5 Alexa Fluor® 488
Alternate Names	SMC1A SMCB SMC1alpha DXS423E DKFZp686L19178 SMC1 SMC1L1 Smcb OTTHUMP00000061876 KIAA0178 SB1.8 MGC138332 CDLS2 Sb1.8
Background Information	SMC (structural maintenance of chromosomes) proteins are Involved in chromosome cohesion during cell cycle and in DNA repair. ATM phosphorylates SMC1 on Ser957 and Ser966 both in vitro and in vivo. Phosphorylated SMC1 is part of the complex that functions as a downstream effector in both the ATM/NBS1 branch and the ATR/MSH2 branch of S-phase checkpoint.

Product Information
Format	AlexaFluor®488
Control	HeLa Cells
Presentation	Purified mouse monoclonal IgG1 conjugated to Alexa Flour® 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Quality Level	MQ100

Applications
Application	This Anti-phospho-SMC1 (Ser957) Antibody, clone 5D11G5 Alexa Fluor 488 is validated for use in WB, FC for the detection of phospho-SMC1 (Ser957).
Key Applications	Western Blotting Flow Cytometry

Biological Information
Immunogen	KLH-conjugated, synthetic peptide corresponding to human SMC1 phosphorylated at Ser957
Epitope	Phosphorylated at and around Ser957
Clone	5D11G5
Host	Mouse
Specificity	Antibody recognizes SMC1 phosphorylolated at Ser957.
Isotype	IgG1
Species Reactivity	Human Xenopus Bovine
Species Reactivity Note	Tested and passed on Human. Predicted to cross-react with xenopus and bovine based on sequence homology
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_006306
Entrez Gene Summary	Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1L2 or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation.
Gene Symbol	SMC1A SMCB SMC1alpha DXS423E DKFZp686L19178 SMC1 SMC1L1 Smcb OTTHUMP00000061876 KIAA0178 SB1.8 MGC138332 CDLS2 Sb1.8
Modifications	Phosphorylation
Purification Method	Protein G Purified
UniProt Number	Q14683
UniProt Summary	FUNCTION: SwissProt: Q14683 # Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint. SIZE: 1233 amino acids; 143233 Da SUBUNIT: Interacts with POLE. Interacts with SYCP2. Interacts with BRCA1. Found in a complex with CDCA5, SMC3 and RAD21, PDS5A/APRIN and PDS5B/SCC-112 (By similarity). Forms a heterodimer with SMC3 in cohesin complexes. Cohesin complexes are composed of the SMC1 (SMC1A or SMC1B) and SMC3 heterodimer attached via their hinge domain, RAD21 which link them, and one STAG protein (STAG1, STAG2 or STAG3), which interacts with RAD21. In germ cell cohesin complexes, SMC1A is mutually exclusive with SMC1B. Interacts with BRCA1. Interacts with KNTC2. SUBCELLULAR LOCATION: Nucleus. Note=Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex. The phosphorylated form on Ser-957 and Ser-966 associates with chromatin during G1/S/G2 phases but not during M phase, suggesting that phosphorylation does not regulate cohesin function. Integral component of the functional centromere-kinetochore complex at the kinetochore region during mitosis. DOMAIN: SwissProt: Q14683 The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC3, forming a V- shaped heterodimer. The two heads of the heterodimer are then connected by different ends of the cleavable RAD21 protein, forming a ring structure (By similarity). PTM: Phosphorylated by ATM upon ionizing radiation in a NBS1- dependent manner. Phosphorylated by ATR upon DNA methylation in a MSH2/MSH6-dependent manner. Phosphorylation of Ser-957 and Ser-966 activates it and is required for S-phase checkpoint activation. DISEASE: SwissProt: Q14683 # Defects in SMC1A are the cause of Cornelia de Lange syndrome type 2 (CDLS2) [MIM:300590]; also known as Cornelia de Lange syndrome X-linked. CDLS is a clinically heterogeneous developmental disorder associated with malformations affecting multiple systems. CDLS is characterized by facial dysmorphisms, abnormal hands and feet, growth delay, cognitive retardation and various other malformations including gastroesophageal dysfunction and cardiac, ophthalmologic and genitourinary anomalies. SIMILARITY: Belongs to the SMC family. SMC1 subfamily.
Molecular Weight	150kDa Calculated

Product Usage Statements
Quality Assurance	Evaluated by Flow Cytometry with Hela Cells
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain refrigerated at 2-8°C in undiluted aliquots for up to 6 months from date of receipt. Protect from light.

Packaging Information
Material Size	100 tests

Global Trade Item Number
Catalogue Number	GTIN
FCMAB108A4	04053252472855

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