Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

09-433 Anti-phospho-IRS1 (Tyr628) mouse/ (Tyr632) human Antibody

Anti-phospho-IRS1 (Tyr628) mouse/ (Tyr632) human Antibody is an antibody against phospho-IRS1 (Tyr628) mouse/ (Tyr632) human for use in WB.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Insulin/Energy Signaling Gene Symbol: IRS1, IRS-1, HIRS-1 UniProt Number: P35568
View Products on Sigmaaldrich.com
09-433
100 µL  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbPurifiedPolyclonal Antibody
Description
Catalogue Number09-433
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-phospho-IRS1 (Tyr628) mouse/ (Tyr632) human Antibody
Alternate Names
  • HIRS-1
  • Insulin Receptor Substrate 1
Background InformationInsulin receptor substrate (IRS) molecules are key mediators in insulin signaling and play a central role in maintaining basic cellular functions such as growth, survival, and metabolism. They act as docking proteins between the insulin receptor and a complex network of intracellular signaling molecules containing Src homology 2 (SH2) domains. Four members (IRS-1, IRS-2, IRS-3, IRS-4) of this family have been identified that differ as to tissue distribution, subcellular localization, developmental expression, binding to the insulin receptor, and interaction with SH2 domain-containing proteins. Results from targeted disruption of the IRS genes in mice have provided important clues to the functional differences among these related molecules, suggesting they play different and specific roles in vivo. The available data are consistent with the notion that IRS-1 and IRS-2 are not functionally interchangeable in tissues that are responsible for glucose production (liver), glucose uptake (skeletal muscle and adipose tissue), and insulin production (pancreatic β cells). In fact, IRS-1 appears to have its major role in skeletal muscle whereas IRS-2 appears to regulate hepatic insulin action as well as pancreatic β cell development and survival. By contrast, IRS-3 and IRS-4 genes appear to play a redundant role in the IRS signaling system. Defects in muscle IRS-1 expression and function have been reported in insulin-resistant states such as obesity and type 2 diabetes.
References
Product Information
FormatPurified
PresentationAffinity purified rabbit polyclonal IgG in storage buffer containing PBS with 0.05% sodium azide in 50% glycerol.
Quality LevelMQ100
Applications
ApplicationAnti-phospho-IRS1 (Tyr628) mouse/ (Tyr632) human Antibody is an antibody against phospho-IRS1 (Tyr628) mouse/ (Tyr632) human for use in WB.
Key Applications
  • Western Blotting
Biological Information
Immunogenpeptide corresponding to amino acids encompassing and including phosphorylated Tyr628 in mouse and Tyr632 in human of IRS1
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes phospho-IRS1 (Tyr628) mouse/ (Tyr632) human
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteTested on human. Predicted to cross react with mouse and rat based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • IRS1
  • IRS-1
  • HIRS-1
Modifications
  • Phosphorylation
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P35568 # May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity).
SIZE: 1242 amino acids; 131591 Da
SUBUNIT: Interacts with the NPXY motif of tyrosine-phosphorylated IGF1R and INSR via the PTB domain. Binds to phosphatidylinositol 3-kinase p85 subunit via the phosphorylated YXXM motifs. Binds ROCK1. Binds to UBTF and PIK3CA in nuclear extracts (By similarity). Interacts with SOCS7.
PTM: Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor (By similarity). & Phosphorylation of Tyr-896 is required for GRB2-binding (By similarity).DISEASE:SwissProt: P35568 # Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
SIMILARITY: SwissProt: P35568 ## Contains 1 IRS-type PTB domain. & Contains 1 PH domain.
Molecular Weight170 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by western blot on lysates from untreated and insulin-treated (30 minutes) HEK 293 cells
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
09-433 04053252742637

Documentation

Anti-phospho-IRS1 (Tyr628) mouse/ (Tyr632) human Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-phospho-IRS1 (Tyr628) mouse/ (Tyr632) human Antibody Certificates of Analysis

TitleLot Number
Anti-phospho-IRS1 (Tyr628) mouse/ (Tyr632) human - 2454705 2454705
Anti-phospho-IRS1 (Tyr628) mouse/ (Tyr632) human - 2466943 2466943
Anti-phospho-IRS1 (Tyr628) mouse/ (Tyr632) human - 2469369 2469369
Anti-phospho-IRS1 (Tyr628) mouse/ (Tyr632) human - 3190910 3190910
Anti-phospho-IRS1 (Tyr628) mouse/ (Tyr632) human - 3420899 3420899
Anti-phospho-IRS1 (Tyr628) mouse/ (Tyr632) human - 3607594 3607594
Anti-phospho-IRS1 (Tyr628) mouse/ (Tyr632) human - 3907053 3907053
Anti-phospho-IRS1 (Tyr628) mouse/ (Tyr632) human - 4040474 4040474
Anti-phospho-IRS1 (Tyr628) mouse/ (Tyr632) human - DAM1506928 DAM1506928
Anti-phospho-IRS1 (Tyr628) mouse/ (Tyr632) human Polyclonal Antibody 3023592
Show more

Related Products & Applications

Related Products

Catalogue Number Description
04-784 Anti-IRS1 Antibody, clone AW58, rabbit monoclonal
05-1085 Anti-IRS1 Antibody, clone 4.2.2
05-1086 Anti-phospho-IRS1 (Ser302 mouse/ Ser307 human) Antibody, clone 8.1.2
05-1087 Anti-phospho-IRS1 (Ser307 mouse/ Ser312 human) Antibody, clone 24.6.2
06-248 Anti-IRS1 Antibody
09-432 Anti-phospho-IRS1 (Tyr608) mouse/ (Tyr612) human Antibody

Categories

Life Science Research > Antibodies and Assays > Primary Antibodies