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OP43A Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) Agarose Conjugate

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OP43A
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
Fe, HMMonoclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~53 kDa wild-type and mutant p53 protein in A431 cells and breast carcinoma tissue.
Catalogue NumberOP43A
Brand Family Calbiochem®
References
ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
Legros, Y., et al. 1994. Oncogene 9, 2071.
Barak, Y., et al. 1993. EMBO J. 12, 461.
Kastan, M.B., et al. 1992. Cell 71, 587.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
Lane, D.P. 1992. Nature 358, 15.
Kastan, M.B., et al. 1991 Cancer Res. 51, 6304.
Product Information
FormLiquid slurry
FormulationAgarose suspension in PBS.
Negative controlSK-OV-3 cells
Positive controlA431 cells
Preservative≤ 0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesImmunoprecipitation Loganzo, F., et al. 1994. Mol. Cell. Different. 2, 23. Epitope Legros, Y., et al. 1994. Oncogene 9, 2071. Original Clone Vojtesek, B., et al. 1992. J. Immunol. Meth. 151, 237.
Key Applications Affinity Chromatography
Immunoprecipitation
Application NotesAffinity Chromatography (see comments)
Immunoprecipitation (15 µl beads/sample; see application references)
Application CommentsRecognizes both mutant and wild-type p53 under denaturing and non-denaturing conditions. This antibody reacts weakly with rodent p53; we do not recommend it for rodent samples. Wild-type p53 has a short half life and is present in low amounts in cells. For immunoprecipitation increasing the amount of sample and labeling with 35S-Met for less than or equal to 1 h will aid in visualizing wild-type p53. Agarose conjugate has also been reported to work with affinity chromotography. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenwild type, recombinant, human p53
ImmunogenHuman
EpitopeWithin amino acids 21-25 of human p53
CloneDO-1
HostMouse
IsotypeIgG2a
Species Reactivity
  • Feline
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
OP43A 0

Documentation

Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) Agarose Conjugate SDS

Title

Safety Data Sheet (SDS) 

Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) Agarose Conjugate Certificates of Analysis

TitleLot Number
OP43A

References

Reference overview
El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
Legros, Y., et al. 1994. Oncogene 9, 2071.
Barak, Y., et al. 1993. EMBO J. 12, 461.
Kastan, M.B., et al. 1992. Cell 71, 587.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
Lane, D.P. 1992. Nature 358, 15.
Kastan, M.B., et al. 1991 Cancer Res. 51, 6304.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision03-October-2007 RFH
ApplicationAffinity Chromatography (see comments)
Immunoprecipitation (15 µl beads/sample; see application references)
DescriptionImmunoaffinity purified mouse monoclonal antibody, generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with X63AG8.653 mouse myeloma cells (see application references). Recognizes the ~53 kDa mutant and wild-type forms of p53.
BackgroundThe human p53 tumor suppressor gene encodes a 393 amino acid phosphoprotein that exhibits sequence-specific DNA binding and directly interacts with various cellular and viral proteins. p53 is the most commonly mutated gene in human cancer, with the majority of the mutations being amino acid substitutions. The normal function of p53 is to effect cell cycle arrest at the G1 and G2 checkpoints in response to DNA damage. This checkpoint function is executed by accumulation of p53 followed by induction of the GADD45, WAF1, and MDM2 genes. The current model of p53 function postulates that p53 senses DNA damage and arrests the cell cycle in either the G1 or G2 phases to allow DNA repair to take place. If repair is not successful, p53 initiates programmed cell death.
HostMouse
Immunogen speciesHuman
Immunogenwild type, recombinant, human p53
EpitopeWithin amino acids 21-25 of human p53
CloneDO-1
IsotypeIgG2a
Speciesfeline, human, not mouse, not rat
Positive controlA431 cells
Negative controlSK-OV-3 cells
FormLiquid slurry
FormulationAgarose suspension in PBS.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤ 0.1% sodium azide
CommentsRecognizes both mutant and wild-type p53 under denaturing and non-denaturing conditions. This antibody reacts weakly with rodent p53; we do not recommend it for rodent samples. Wild-type p53 has a short half life and is present in low amounts in cells. For immunoprecipitation increasing the amount of sample and labeling with 35S-Met for less than or equal to 1 h will aid in visualizing wild-type p53. Agarose conjugate has also been reported to work with affinity chromotography. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
Legros, Y., et al. 1994. Oncogene 9, 2071.
Barak, Y., et al. 1993. EMBO J. 12, 461.
Kastan, M.B., et al. 1992. Cell 71, 587.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
Lane, D.P. 1992. Nature 358, 15.
Kastan, M.B., et al. 1991 Cancer Res. 51, 6304.
Application referencesImmunoprecipitation Loganzo, F., et al. 1994. Mol. Cell. Different. 2, 23. Epitope Legros, Y., et al. 1994. Oncogene 9, 2071. Original Clone Vojtesek, B., et al. 1992. J. Immunol. Meth. 151, 237.