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04-1479 Anti-methyl-PP2A Antibody, C subunit, clone 2A10

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04-1479
150 µg  
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Overview

Replacement Information
Replacement Information04-1479 is a recommended replacement for 05-546.

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, Yeast (S. cerevisiae), R, Po, Rb, Ch, DrWBMPurifiedMonoclonal Antibody
Description
Catalogue Number04-1479
Replaces05-546
DescriptionAnti-methyl-PP2A Antibody, C subunit, clone 2A10
Background InformationType 2 protein serine/threonine phosphatase (PP2A) is a trimer composed of a catalytic C subunit and two regulatory (A and B) subunits; many isoforms for the A and B subunits have been found. PP2A has a high specific activity against the α subunit of phosphorylase kinase and is insensitive to Inhibitor-2. PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and by its high sensitivity to inhibition by okadaic acid. It is also thought to be the principal down-modulator of serine/threonine kinase cascades. PP2B requires Ca2+/calmodulin for activity, and is an activator of NFAT transcription factors. PP2C requires Mg2+, but is insensitive to okadaic acid, microcystin LR, or the calmodulin inhibitor trifluoperazine.
Product Information
FormatPurified
Control
  • Lysates from a yeast wild type strain (strain lacking the methyltransferase, delta PPM1) and a strain lacking the methylestrase, delta PPE1.
PresentationPurified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl and 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-methyl-PP2A Antibody, C subunit, clone 2A10 is validated for use in WB for the detection of methyl-PP2A.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated, synthetic peptide (C-βA-RRTPDYFL-Ome) corresponding to amino acids 302-309 of human PP2A catalytic subunit. Clone 2A10.
Clone2A10
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityRecognizes the methylated form of PP2A, catalytic subunit.
IsotypeIgG1κ
Species Reactivity
  • Human
  • Mouse
  • Yeast (S. cerevisiae)
  • Rat
  • Pig
  • Rabbit
  • Chicken
  • Drosophila
Species Reactivity NoteHuman, mouse and yeast. Predicted to cross react with rat, pig, rabbit, chicken and Drosophila.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Modifications
  • Methylation
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~36 kDa
Product Usage Statements
Quality AssuranceEvaluated by western blot on lysates from a yeast wild type strain (strain lacking the methyltransferase, delta PPM1) and a strain lacking the methylestrase, delta PPE1.

Western Blot Analysis: 1-5 µg/mL of this antibody detected the methylated form of PP2A catalytic subunit in yeast lysates lacking the methylestrase (increased methylation). Treatment of the blot with 0.2 M NaOH will demethylate proteins, thus eliminating detection of PP2A.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Packaging Information
Material Size150 µg
Global Trade Item Number
Catalogue Number GTIN
04-1479 04053252477546