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ABE430 Anti-methyl-CARM1 (Arg551) Antibody

This Anti-methyl-CARM1 (Arg551) | ABE430 antibody is validated for use in WB, IP for the detection of methyl-CARM1 (Arg551) | ABE430.

Anti-methyl-CARM1 (Arg551) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: CARM1, PRMT4 UniProt Number: Q86X55
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ABE430
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IPRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE430
DescriptionAnti-methyl-CARM1 (Arg551) Antibody
Alternate Names
  • Histone-arginine methyltransferase CARM1
  • Coactivator-associated arginine methyltransferase 1
  • Protein arginine N-methyltransferase 4
Background InformationCARM1, also known as Histone-arginine methyltransferase CARM1, Coactivator-associated arginine methyltransferase 1, Protein arginine N-methyltransferase 4, and encoded by the gene name CARM1 and PRMT4, catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to arginine residues. CARM1 methylates (mono- and asymmetric dimethylation) the the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Upon gene activation CARM1 together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs. CARM1 is overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide with 10% glycerol.
Quality LevelMQ100
Applications
ApplicationThis Anti-methyl-CARM1 (Arg551) | ABE430 antibody is validated for use in WB, IP for the detection of methyl-CARM1 (Arg551) | ABE430.
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application NotesImmunoprecipitation Analysis: A representative lot immunoprecipitated methyl-CARM1 (Arg551) in vitro methylation of a control buffer, 15 mg nuclear lysates, and 15 mg cytoplasmic lysates from CARM1/MEF cells by 1 mg CARM1 WT and CARM1 R551K (Kuhn, P., et al. (2011). Nucleic Acids Research. 39(7):2717-2726).
Biological Information
ImmunogenLinear peptide corresponding to human CARM1 (Arg551) methylated at R551.
EpitopeMethylated R551
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • CARM1
  • PRMT4
Modifications
  • Methylation
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~62 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293T CARM1 KO cells restored with WT CARM1, but not in HEK293T CARM1 KO cells restored with R551K mutant CARM1 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected methyl-CARM1 (Arg551) in 10 µg of HEK293T CARM1 KO cells restored with WT CARM1, but not in HEK293T CARM1 KO cells restored with R551K mutant CARM1 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE430 04055977125283