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OP55 Anti-c-Jun/AP-1 (Ab-3) Mouse mAb (76)

Anti-c-Jun/AP-1 (Ab-3) Mouse mAb (76) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~39 kDa c-Jun/AP-1 protein in proliferating cells and most tumor tissues.
Catalogue NumberOP55
Brand Family Calbiochem®
Application Data
Detection of human c-jun by immunoblotting. Sample: Whole cell lysates (20 μg) from MCF7 cells. Primary antibody: Anti-c-Jun/AP-1 (Ab-3) Mouse mAb (76) (Cat. No. OP55) (2.5 μg/ml). Detection: chemiluminescence
References
ReferencesVolm, M., et al. 1993. Clin. Exp. Metastasis 11, 325.
Angel, P., et al. 1988. Nature 332, 166.
Rouscher III, F.J., et al. 1988. Science 240, 1010.
Sassone-Corsi, P., et al. 1988. Cell 54, 553.
Bohmann, D., et al. 1987. .Science 238, 1386.
Vogt, P.K., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 3316.
Arndt, K., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 8516.
Cavaleri, F., et al. 1985. Virology 143, 680.
Product Information
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 50% glycerol.
Negative controlQuiescent cells
Positive controlGrowing cells (such as HeLa) or breast or colon tissue
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesImmunoblotting (2.5 µg/ml)
Paraffin Sections (2.5 µg/ml, pressure cooker pre-treatment required)
Application CommentsThis is the preferred c-Jun antibody for immunoblotting. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant jun protein
Clone76
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
OP55 0

Documentation

Anti-c-Jun/AP-1 (Ab-3) Mouse mAb (76) SDS

Title

Safety Data Sheet (SDS) 

Anti-c-Jun/AP-1 (Ab-3) Mouse mAb (76) Certificates of Analysis

TitleLot Number
OP55

References

Reference overview
Volm, M., et al. 1993. Clin. Exp. Metastasis 11, 325.
Angel, P., et al. 1988. Nature 332, 166.
Rouscher III, F.J., et al. 1988. Science 240, 1010.
Sassone-Corsi, P., et al. 1988. Cell 54, 553.
Bohmann, D., et al. 1987. .Science 238, 1386.
Vogt, P.K., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 3316.
Arndt, K., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 8516.
Cavaleri, F., et al. 1985. Virology 143, 680.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision03-October-2007 RFH
ApplicationImmunoblotting (2.5 µg/ml)
Paraffin Sections (2.5 µg/ml, pressure cooker pre-treatment required)
Application Data
Detection of human c-jun by immunoblotting. Sample: Whole cell lysates (20 μg) from MCF7 cells. Primary antibody: Anti-c-Jun/AP-1 (Ab-3) Mouse mAb (76) (Cat. No. OP55) (2.5 μg/ml). Detection: chemiluminescence
DescriptionPurified mouse monoclonal antibody. Recognizes the ~32-39 kDa c-Jun/AP-1 protein.
BackgroundThe jun gene is a cell derived sequence that has been identified as the presumed oncogene of avian sarcoma virus 17, which induces fibrosarcomas in chickens. v-Jun shares homology with the DNA binding domain of yeast transcription factor GCN4. Cloning of the proto-oncogene c-Jun demonstrated that it encoded a DNA binding protein of ~39 kDa with structural and functional properties of transcription factor AP-1. The oncogene protein product of Fos can bind to c-Jun. The Fos/Jun protein complex binds specifically to a sequence element in DNA referred to as the AP-1 binding site. In some studies, overexpression of the c-Jun protein has been correlated with increased metastatic potential.
HostMouse
Immunogenrecombinant jun protein
Clone76
IsotypeIgG₁
Specieshuman, mouse, rat
Positive controlGrowing cells (such as HeLa) or breast or colon tissue
Negative controlQuiescent cells
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 50% glycerol.
Concentration Label Please refer to vial label for lot-specific concentration
PreservativeNone
CommentsThis is the preferred c-Jun antibody for immunoblotting. Antibody should be titrated for optimal results in individual systems.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesVolm, M., et al. 1993. Clin. Exp. Metastasis 11, 325.
Angel, P., et al. 1988. Nature 332, 166.
Rouscher III, F.J., et al. 1988. Science 240, 1010.
Sassone-Corsi, P., et al. 1988. Cell 54, 553.
Bohmann, D., et al. 1987. .Science 238, 1386.
Vogt, P.K., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 3316.
Arndt, K., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 8516.
Cavaleri, F., et al. 1985. Virology 143, 680.