Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

ABC1387 Anti-Zyxin

Anti-Zyxin Antibody, Cat. No. ABC1387, is a rabbit polyclonal antibody that detects Zyxin in human and murine cells and has been tested for use in Immunocytochemistry and Western Blotting.

Anti-Zyxin MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: ZYX UniProt Number: Q15942
View Products on Sigmaaldrich.com
ABC1387
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MICC, WBRbSerumPolyclonal Antibody
Description
Catalogue NumberABC1387
DescriptionAnti-Zyxin
Alternate Names
  • Zyxin-2
Background InformationZyxin (UniProt: Q15942; also known as Zyxin-2) is encoded by the ZYX gene (Gene ID: 7791) in human. Zyxin is an evolutionarily conserved LIM domain protein that acts as an adhesion plaque protein concentrated at sites of cell adhesion and is widely expressed. It has been implicated in cell adhesion, cell motility, and signaling. Zyxin is reported to associate with members of the Enabled (Ena)/vasodilator-stimulated phosphoprotein (VASP) family of cytoskeletal regulators and plays a role in cytoskeletal dynamics and signaling. Zyxin contains three LIM zinc binding domains (aa 384-443; 444-503; and 504-570), which act as a negative regulator of Zyxin-VASP complexes. Also, it contains four proline rich ActA repeats (aa 64-77; 94-108; 115-121; and 127-137) that are known to interact directly with Ena/VASP proteins. Loss of Zyxin results in inability to thicken stress fibers in response to mechanical stimuli. (Ref.: Hoffman LM et al (2003). Mol. Cell Biol. 23(1); 70-79; Moody JD et al. (2009). Biochem. Biophys. Res. Commun. 378(3), 625-628; Yoshigi et al (2005). J. Cell Biol. 171(2), 209-215.).
References
Product Information
FormatSerum
PresentationRabbit polyclonal antiserum with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Zyxin Antibody, Cat. No. ABC1387, is a rabbit polyclonal antibody that detects Zyxin in human and murine cells and has been tested for use in Immunocytochemistry and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Western Blotting
Application NotesWestern Blotting Analysis: A 1:5,000 dilution from a representative lot detected Zyxin in 10 µg of NIH/3T3 cell lysate.

Immunocytochemistry Analysis: A representative lot detected Zyxin in Immunocytochemistry applications (Hoffman, L.M., et. al. (2003). Mol Cell Biol. 23(1):70-9; Hoffman, L.M., et. al. (2006). J Cell Biol. 172(5):771-82).

Western Blotting Analysis: A representative lot detected Zyxin in Western Blotting applications(Hoffman, L.M., et. al. (2003). Mol Cell Biol. 23(1):70-9; Hoffman, L.M., et. al. (2006). J Cell Biol. 172(5):771-82).

Immunocytochemistry Analysis A 1:600 dilution from a representative lot detected Zyxin in mouse fibroblasts (Courtesy of Dr. Laura Hoffman at Huntsman Cancer Institute, University of Utah).

Western Blotting Analysis: A representative lot detected Zyxin in Western Blotting applications (Moody, J.D., et. al. (2009). Biochem Biophys Res Commun. 378(3):625-8).

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Zyxin in mouse fibroblast, HeLa, and U2OS cell lysates (Courtesy of Dr. Laura Hoffman at Huntsman Cancer Institute, University of Utah).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 20 amino acids from the N-terminal region of human Zyxin.
Epitopeunknown
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Zyxin in human and murine cells. It targets an epitope within 20 amino acids from the N-terminal region.
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse. Predicted to react with Monkey based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • ZYX
Purification MethodUnpurified
UniProt Number
Molecular Weight~75 kDa observed; 61.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Zyxin in 10 µg of A431 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABC1387 04054839357947