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ABT79 Anti-WASH complex subunit FAM21C Antibody

Anti-WASH complex subunit FAM21C Antibody detects level of WASH complex subunit FAM21C & has been published & validated for use in WB & IC.

Anti-WASH complex subunit FAM21C Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeletal Signaling Gene Symbol: FAM21C, FAM21A, VPEF2 UniProt Number: Q9Y4E1
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ABT79
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, ICCRbPurifiedPolyclonal Antibody
Description
Catalogue NumberABT79
DescriptionAnti-WASH complex subunit FAM21C Antibody
Alternate Names
  • family with sequence similarity 21, member C
  • vaccinia virus penetration factor
  • WASH complex subunit FAM21C
Background InformationFAM21C, also known as WASH complex subunit FAM21C, is a part of the a 500 kDa core complex that allows the Wiskott-Aldrich syndrome protein (WASH) function. FAM21C can be found in the membranes of early endosomes. FAM21C cooperates with CAPZ and hinders its actin-capping activity, links WASH to endosomes, and is required for WASH-dependent retromer-mediated sorting.
References
Product Information
FormatPurified
Control
  • HEK293 cell lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-WASH complex subunit FAM21C Antibody detects level of WASH complex subunit FAM21C & has been published & validated for use in WB & IC.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:500 dilutioin from a previous lot detected WASH complex subunit FAM21C in NIH/3T3, A431, and HeLa cells.
Biological Information
ImmunogenRecombinant protein corresponding to human WASH complex subunit FAM21C.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
Species Reactivity
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • FAM21C
  • FAM21A
  • VPEF2
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: Component of the WASH complex, a complex present at the surface of endosomes that recruits and activates the Arp2/3 complex to induce actin polymerization. The WASH complex plays a key role in the fission of tubules that serve as transport intermediates during endosome sorting. In the complex, it probably mediates the recruitment of the complex to endosome membranes.

SUBUNIT STRUCTURE: Component of the WASH complex, composed of F-actin-capping protein subunit alpha (CAPZA1, CAPZA2 or CAPZA3), F-actin-capping protein subunit beta (CAPZB), WASH (WASH1, WASH2P, WASH3P, WASH4P, WASH5P or WASH6P), FAM21 (FAM21A, FAM21B or FAM21C), KIAA1033, KIAA0196 and CCDC53. Interacts (via N-terminus) with WASH1; the interaction is direct.

SUBCELLULAR LOCATION: Early endosome membrane

MISCELLANEOUS: In human, FAM21 has undergone evolutionary duplication, with 4 highly homologous family members existing, including FAM21A, FAM21B, FAM21C, and an N-terminally truncated FAM21D form.

SEQUENCE SIMILARITIES: Belongs to the FAM21 family.

SEQUENCE CAUTION: The sequence AAH06456.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAA25518.1 differs from that shown. Reason: Erroneous initiation.
Molecular Weight ~145 kDa observed
Non-specific bands may be observed >200 kDa and at ~10 kDa.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HEK293 cell lysate.

Western Blot Analysis: 5 µg/mL of this antibody detected WASH complex subunit FAM21C in 10 µg of HEK293 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABT79 04053252645372

Documentation

Anti-WASH complex subunit FAM21C Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-WASH complex subunit FAM21C Antibody Certificates of Analysis

TitleLot Number
Anti-WASH complex subunit FAM21C - 2140074 2140074
Anti-WASH complex subunit FAM21C - 2424801 2424801
Anti-WASH complex subunit FAM21C - 2438675 2438675
Anti-WASH complex subunit FAM21C - 1951091 1951091
Anti-WASH complex subunit FAM21C - 2211056 2211056
Anti-WASH complex subunit FAM21C - 2301425 2301425
Anti-WASH complex subunit FAM21C - 3270776 3270776
Anti-WASH complex subunit FAM21C - 3494929 3494929
Anti-WASH complex subunit FAM21C - 3560681 3560681
Anti-WASH complex subunit FAM21C - 3807960 3807960
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