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MAB3574-25UG Anti-Vinculin, clone VIIF9 (7F9)

Anti-Vinculin Antibody, clone VIIF9 (7F9) is an antibody against Vinculin for use in IP, WB, IC, IH(P).

Anti-Vinculin, clone VIIF9 (7F9) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Cytoskeletal Signaling Gene Symbol: VCL, MVCL, Vinculin, Metavinculin, OTTHUMP00000043885, CMD1W, vinculin UniProt Number: P18206
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MAB3574-25UG
25 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
B, H, M, Mk, Po, RbIP, WB, ICC, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB3574-25UG
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Vinculin, clone VIIF9 (7F9)
References
Product Information
FormatPurified
Control
  • POSITIVE CONTROL:

    Vinculin is present in all cell types.
PresentationLiquid in 0.02M Phosphate buffer with 0.25M NaCl and 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Vinculin Antibody, clone VIIF9 (7F9) is an antibody against Vinculin for use in IP, WB, IC, IH(P).
Key Applications
  • Immunoprecipitation
  • Western Blotting
  • Immunocytochemistry
  • Immunohistochemistry (Paraffin)
Application NotesWestern blot. 1:1000; ~117kDa band. Suggested blocking buffer is TBS-Tween with 2% BSA. Suggested dilution buffer is TBS-Tween with 0.05% sodium azide. Preferred gel percentage is 10% and/or 4-20% (or similar) gradient gel.

Immunohistochemistry on frozen and paraffin embedded tissue sections. Suggested fixation for frozen tissue sections is acetone fix for 6 minutes at room temperature. For formalin fixed paraffin embedded tissue sections: microwave in 0.01M citrate buffer (pH 6.0) for 8-10 minutes (note that all microwaves differ and adjustments may need to be made). If necessary follow with enzyme digestion (0.01% pronase for 10 mintues). Suggested blocking agent is fetal bovine serum. The antibody has also been used successfully on methyl-Carnoy fixed tissue.

Immunocytochemistry (see application notes below).

Immunoprecipitation. Suggested extraction buffer is 20 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% Triton X-100, 0.1% SDS, 0.5% deoxycholic acid-NaCl and 0.5 mM PMSF. Final reaction volume is 1 mL and suggested capture agent is agarose conjugated anti-mouse IgG. Note that the muscle-specific isoform, meta-vinculin (MW 150 kDa) will co-precipitate.

Optimal working dilutions must be determined by the end user.

APPLICATION NOTES FOR MAB3574

IMMUNOCYTOCHEMISTRY

For cultured cells, use the following method: 1) wash cells twice with buffer "A" (100 mM Pipes (to make it dissolve, titrate with KOH), 0.5mM MgCl2, 0.1 mM EDTA, 0.01M EGTA (to make it dissolve, titrate with KOH), pH 6.9); 2) fix 15 min @RT (3.7% formalin, 0.2% Triton X-100, 2M Glycerol, prepared on buffer A); 3) wash 3 times with buffer "A"; 4) proceed with incubation with primary Abs, etc.
Biological Information
ImmunogenCrude smooth muscle extract from normal human adult uterus.
CloneVIIF9 (7F9)
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityVinculin. Cellular localization is Intracellular, associated with cytoskeleton.
IsotypeIgG1
Species Reactivity
  • Bovine
  • Human
  • Mouse
  • Monkey
  • Pig
  • Rabbit
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryVinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. Human vinculin protein exhibits a greater than 95% sequence identity to the chicken vinculin protein.
Gene Symbol
  • VCL
  • MVCL
  • Vinculin
  • Metavinculin
  • OTTHUMP00000043885
  • CMD1W
  • vinculin
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P18206 # Involved in cell adhesion. May be involved in the attachment of the actin-based microfilaments to the plasma membrane. May also play important roles in cell morphology and locomotion.
SIZE: 1134 amino acids; 123799 Da
SUBUNIT: Exhibits self-association properties. Interacts with NRAP and SORBS1 (By similarity). Interacts with TLN1.
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. Cell junction, adherens junction; Cytoplasmic side. Note=Cytoplasmic face of adhesion plaques.
TISSUE SPECIFICITY: Metavinculin is muscle-specific.
PTM: Phosphorylated on serines, threonines and tyrosines and acylated by myristic acid and/or palmitic acid.
DISEASE:SwissProt: P18206 # Defects in VCL are a cause of hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM).
SIMILARITY: SwissProt: P18206 ## Belongs to the vinculin/alpha-catenin family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceTested
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at 2-8°C in undiluted aliquots up to 6 months after date of receipt
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB3574-25UG 04054839350375