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ABS2215-100UG Anti-Uromodulin/THP

This rabbit polyclonal Anti-Uromodulin / THP, Cat. No. ABS2215 is tested for use in Western Blotting and Immunohistochemistry (Paraffin), for the detection of Human Uromodulin (Tamm-Horsfall protein, THP).

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ABS2215-100UG
100 μg  
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Overview

Replacement Information
Description
Catalogue NumberABS2215-100UG
DescriptionAnti-Uromodulin/THP
Alternate Names
  • Tamm-Horsfall urinary glycoprotein
  • THP
Background InformationUromodulin (UniProt: P07911; also known as Tamm-Horsfall urinary glycoprotein, THP) is encoded by the UMOD gene (Gene ID: 7369) in human. Uromodulin is a homodimeric glycoprotein that is produced in the kidney and can polymerize into long filaments. It is expressed in the tubular cells of the kidney and is most abundant protein in normal urine. It plays a role in biogenesis and organization of the apical membrane of epithelial cells of the thick ascending limb of Henle's loop, where it promotes formation of complex filamentous gel-like structure that may play a role in the water barrier permeability. It also facilitates neutrophil migration across renal epithelia. Uromodulin contains a ZP domain (aa 334-589) that mediates polymerization, leading to the formation of long filaments. The core of the filament consists of stacked ZP domains, which assemble into a helical structure. Each ZP domain consists of an N-terminal (ZP-N; aa 334-429) and C-terminal (ZP-C; aa 465-589) region connected by a flexible linker. The linker allows the ZP domain to wrap around the ZP-C subdomain of the preceding subunit. It is proteolytically cleaved at a conserved C-terminal proteolytic cleavage site to generate the secreted form found in the urine. In the urine, it may contribute to colloid osmotic pressure, retard passage of positively charged electrolytes, and prevent urinary tract infection. It also inhibits the formation of liquid containing supersaturated salts and subsequent formation of salt crystals. Five isoforms of uromodulin have been described that are produced by alternative splicing. (Ref.: Micanovic, R., et al. (2022). Am. J. Physiol. Renal Physiol. 322(4); F403-418; Schmid, M., et al. (2010). Cell Physiol. Biochem. 26(3); 311-318; Santambrogio, S., et al. (2008). Biochem. Biophys. Res. Commun. 370(3); 410-413).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in PBS with 0.02% sodium azide.
Quality LevelMQ200
Applications
ApplicationThis rabbit polyclonal Anti-Uromodulin / THP, Cat. No. ABS2215 is tested for use in Western Blotting and Immunohistochemistry (Paraffin), for the detection of Human Uromodulin (Tamm-Horsfall protein, THP).
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesTested applications

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Uromodulin/THP in human kidney tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 22 amino acids from the C-terminal region of Human Uromodulin/THP.
EpitopeC-terminal
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Uromodulin/THP. It targets an epitope within 22 amino acids from the C-terminal region.
IsotypeIgGκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • UMOD
Purification MethodPurified
UniProt Number
Molecular Weight~105 kDa observed; 69.76 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Human kidney tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Uromodulin/THP in Human kidney tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABS2215-100UG 04065268978931