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MABT1334-25UL Anti-Tropomyosin-4 Antibody, clone 2D2A12

Anti-Tropomyosin-4, clone 2D2A12, Cat. No. MABT1334, is a mouse monoclonal antibody that detects Tropomyosin delta-4 chain and is tested for use in Western Blotting.

MABT1334-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABT1334-25UL
DescriptionAnti-Tropomyosin-4 Antibody, clone 2D2A12
Alternate Names
  • Tropomyosin delta-4 chain
  • TM-4
Background InformationTropomyosin alpha-4 chain (UniProt: P09495; also known as Tropomyosin-4, TM-4) is encoded by the Tpm4 gene (Gene ID: 24852) in rat. Tropomyosins are dimers of coiled-coil proteins that provide stability to actin filaments and regulate access of other actin-binding proteins. Tropomyosin-4 1 is a cytoskeletal protein that associates with F-actin stress fibers and binds to actin filaments in muscle and non-muscle cells. It plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. It has a coiled coil structure formed by two polypeptide chains. It is abundantly expressed in kidney, lung, and spleen and is also observed in muscle and stomach tissues. Loss of Tpm4.1 is reported to lead to disturbance in cell-cell adhesions and invasive behavior in breast epithelial cells through Rac1-myosin IIB signaling. In colon cancer cells, TPM4 mRNA and protein expressions is shown to be significantly reduced and its low expression correlates with the clinal stage. Clone 2D2A12 detects both rat and human tropomyosin-4 and does not react with other exon1b containing isoforms from other tropomyosins. (Ref.: Schevzov, G., et al. (2011). BioArchitecture, 1 (4); 135-164; Jeong, S., et al. (2017). Oncotarget. 8(20); 33544-33559; Yang, R., et al. (2018). Biomed. Pharmacother. 101; 1-7).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-Tropomyosin-4, clone 2D2A12, Cat. No. MABT1334, is a mouse monoclonal antibody that detects Tropomyosin delta-4 chain and is tested for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected Tropomyosin-4 in Western Blotting applications (Schevzov, G., et. al. (2011). Bioarchitecture. 1(4):135-164).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 19 amino acids from the N-terminal region of rat Tropomysin-4 corresponding to exon 1b in Tpm4 gene.
Clone2D2A12
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 2D2A12 is a mouse monoclonal antibody that detects rat Tropomyosin-4. It targets an epitope within 19 amino acids from the N-terminal region corresponding to exon 1b in Tpm4 gene.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Rat based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Tpm4
Purification MethodProtein G purified
UniProt Number
Molecular Weight~30 kDa observed; 28.51 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in lysates from mouse skeletal muscle (Negative control) and human BJ fibroblasts (Positive control).

Western Blotting Analysis: A 1:500 dilution of this antibody detected Tropomyosin-4 in lysate from human BJ fibroblasts, but not in mouse skeletal muscle extract (Negative control).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABT1334-25UL 04061841844426

Documentation

Anti-Tropomyosin-4 Antibody, clone 2D2A12 SDS

Title

Safety Data Sheet (SDS) 

Anti-Tropomyosin-4 Antibody, clone 2D2A12 Certificates of Analysis

TitleLot Number
Anti-Tropomyosin-4, clone 2D2A12 - Q3438675 Q3438675

References

Reference overviewPub Med ID
Tropomyosin isoforms and reagents
Galina Schevzov 1 , Shane P Whittaker, Thomas Fath, Jim Jc Lin, Peter W Gunning
Bioarchitecture  1(4)  135-164  2011

Show Abstract 		22069507 22069507