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MABC1765-25UL Anti-TfR1 (CD71) Antibody, clone 3F3-FMA

Anti-TfR1 (CD71), clone 3F3-FMA, Cat. No. MABC1765, is a mouse monoclonal antibody that detects Tfr1 and is an excellent ferroptosis marker. It is tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry, and Western Blotting.

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MABC1765-25UL
25 µL  
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Overview

Replacement Information
Description
Catalogue NumberMABC1765-25UL
DescriptionAnti-TfR1 (CD71) Antibody, clone 3F3-FMA
Alternate Names
  • TR
  • TfR
  • TfR1
  • Trfr
  • T9
  • p90
  • CD71
Background InformationTransferrin receptor protein 1 (UniProt: Q62351; also known as TR, TfR, TfR1, Trfr, T9, p90, CD71) is encoded by the Tfrc (also known as Trfr) gene (Gene ID: 7037) in human. TfR1 is a single-pass type II membrane glycoprotein with a cytoplasmic domain (aa 1-67), a transmembrane domain (aa 68-88), and a long extracellular domain (aa 89-760). It can also be proteolytically cleaved at Arg100 to generate the soluble serum form (sTfR). TfR1 is involved in the uptake of iron via receptor mediated endocytosis into specialized endosomes where acidification leads to release of iron. TfR1 is reported to be essential for erythrocyte development and positive regulation of T and B cell proliferation through iron uptake. It also acts as a lipid sensor that regulates mitochondrial fusion by regulating the activation of the JNK pathway and degradation of mitofusin MFN2. Under conditions of low dietary levels of stearate (C18:0), it promotes the activation of JNK pathway that results in HUWE1-mediated ubiquitination and degradation of mitofusin MFN2 and inhibition of mitochondrial fusion. Clone 3F3-FMA is reported to be an effective ferroptosis-staining agent. It stains plasma membrane and the perinuclear region associated with the Golgi and the endosomal recycling compartment. (Ref.: Senilymaz, D., et al. (2015). Nature. 525(7567); 124-128; Feng, H., et al. (2020). Cell Rep. 30(10); 3411-3423).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-TfR1 (CD71), clone 3F3-FMA, Cat. No. MABC1765, is a mouse monoclonal antibody that detects Tfr1 and is an excellent ferroptosis marker. It is tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry, and Western Blotting.
Key Applications
  • Flow Cytometry
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blotting
Application NotesFlow Cytometry Analysis: A representative lot detected TfR1 (CD71) in Flow Cytometry applications (Feng, H., et. al. (2020). Cell Rep. 30(10): 3411-3423).

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected TfR1 (CD71) in HT-1080 cells.

Immunohistochemistry Applications: A representative lot detected TfR1 (CD71) in Immunohistochemistry applications (Feng, H., et. al. (2020). Cell Rep. 30(10): 3411-3423).

Immunocytochemistry Analysis: A representative lot detected TfR1 (CD71) in Immunocytochemistry applications (Feng, H., et. al. (2020). Cell Rep. 30(10): 3411-3423).

Western Blotting Analysis: A representative lot detected TfR1 (CD71) in Western Blotting applications (Feng, H., et. al. (2020). Cell Rep. 30(10): 3411-3423).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenPurified membrane fractions from OCI-LY7 cells.
EpitopeUnknown
Clone3F3-FMA
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 3F3-FMA is a mouse monoclonal antibody that detects human Transferrin receptor protein 1.
IsotypeIgG1κ
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Tfrc
  • Trfr
Purification MethodProtein G purified
UniProt Number
Molecular Weight~90 kDa observed; 84.87 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HT-1080 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected TfR1 (CD71) in HT-1080 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABC1765-25UL 04065265944670

Documentation

Anti-TfR1 (CD71) Antibody, clone 3F3-FMA SDS

Title

Safety Data Sheet (SDS) 

Anti-TfR1 (CD71) Antibody, clone 3F3-FMA Certificates of Analysis

TitleLot Number
Anti-TfR1 (CD71), clone 3F3-FMA - 3794324 3794324
Anti-TfR1 (CD71), clone 3F3-FMA - 3800107 3800107
Anti-TfR1 (CD71), clone 3F3-FMA - 3802600 3802600
Anti-TfR1 (CD71), clone 3F3-FMA - 3804073 3804073
Anti-TfR1 (CD71), clone 3F3-FMA - 3816718 3816718
Anti-TfR1 (CD71), clone 3F3-FMA - 3819162 3819162
Anti-TfR1 (CD71), clone 3F3-FMA - 3846469 3846469
Anti-TfR1 (CD71), clone 3F3-FMA - 3874863 3874863
Anti-TfR1 (CD71), clone 3F3-FMA - 3884792 3884792
Anti-TfR1 (CD71), clone 3F3-FMA - 3885548 3885548
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