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AP1058 Anti-TOM70 Rabbit pAb

Anti-TOM70 Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Translocase Outer Mitochondrial Membrane

AP1058
  
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Overview

Replacement Information

Key Spec Table

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes the ~70 kDa TOM70 protein in mouse liver mitochondria.
Catalogue NumberAP1058
Brand Family Calbiochem®
SynonymsAnti-Translocase Outer Mitochondrial Membrane
Application Data
Detection of rat TOM70 by immunoblotting: Samples: whole extract (100 µg) from rat liver mitochondria. Primary antibody: Anti-TOM70 Rabbit pAb (Cat. No. AP1058) (0.25 µg/ml). Detection: chemiluminescence. Data courtesy of Sandra Wiley and Matthew Rardin, University of California San Diego.
References
ReferencesLee, J., et al. 2007. Mol. Cell Prot. 4, 669.
Suzuki, H., et al. 2002. J. Cell Sci. 9, 1895.
Endo, T., et al. 2002. Biochim. Biophys. Acta. 1, 3.
Product Information
FormLiquid
FormulationIn PBS.
Positive controlRat liver mitochondria
PreservativeNone
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (0.25 µg/ml)
Application CommentsAntibody should titrated for optimal results in individual sytems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids surrounding Ser⁹⁴ of human TOM70
ImmunogenHuman
HostRabbit
IsotypeIgG
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AP1058 0

Documentation

Anti-TOM70 Rabbit pAb Certificates of Analysis

TitleLot Number
AP1058

References

Reference overview
Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.
Suzuki, H., et al. 2002. J. Cell Sci. 9, 1895.
Endo, T., et al. 2002. Biochim. Biophys. Acta. 1, 3.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision12-May-2008 JSW
SynonymsAnti-Translocase Outer Mitochondrial Membrane
ApplicationImmunoblotting (0.25 µg/ml)
Application Data
Detection of rat TOM70 by immunoblotting: Samples: whole extract (100 µg) from rat liver mitochondria. Primary antibody: Anti-TOM70 Rabbit pAb (Cat. No. AP1058) (0.25 µg/ml). Detection: chemiluminescence. Data courtesy of Sandra Wiley and Matthew Rardin, University of California San Diego.
DescriptionImmunoaffinty purified rabbit polyclonal antibody. Recognizes the ~70 kDa TOM70 protein.
BackgroundThe translocase of outer mitochondrial membrane (TOM) complex is a multisubunit complex involved in the recognition, unfolding, and translocation of preproteins into the mitochondria. TOM20 and TOM70 are the two major receptor subunits of the TOM complex and constitute the binding site for mitochondrial proteins. TOM70 is anchored in the outer membrane by a hydrophobic segment near the N-terminus with the 65 kD C-terminus facing the cytosol. TOM70 forms a dimer and is associated with the TOM complex via interactions with TOM20. TOM70 binds various precursor proteins, including cytochrome c1 and aldehyde dehydrogenase III. Substrates for TOM70 are highly hydrophobic proteins and require molecular chaperones in the cytosol, such as Hsp70 and mitochondrial import stimulating factor (MSF), to maintain themselves in an import-competent state. Phosphorylation of TOM70 has been detected in screens of the mitocohondrial proteome and TOM70 had been shown to be required for proper targeting of the apopoptsis regulator Mcl-1.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide corresponding to amino acids surrounding Ser⁹⁴ of human TOM70
IsotypeIgG
Specieshuman, mouse, rat
Positive controlRat liver mitochondria
FormLiquid
FormulationIn PBS.
Concentration Label Please refer to vial label for lot-specific concentration
PreservativeNone
CommentsAntibody should titrated for optimal results in individual sytems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesLee, J., et al. 2007. Mol. Cell Prot. 4, 669.
Suzuki, H., et al. 2002. J. Cell Sci. 9, 1895.
Endo, T., et al. 2002. Biochim. Biophys. Acta. 1, 3.