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GF10 Anti-TGF-α (Ab-2) Mouse mAb (213-4.4)

This Anti-TGF-α (Ab-2) Mouse mAb (213-4.4) is validated for use in Frozen Sections, Immunocytochemistry, Immunoblotting Paraffin Sections for the detection of TGF-α (Ab-2).

Anti-TGF-α (Ab-2) Mouse mAb (213-4.4) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Transforming Growth Factor-α

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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
Ca, Ht, H, M, RMMonoclonal Antibody
Description
OverviewRecognizes the ~6 kDa TGF-α protein in A2058 cells or skin tissue.
Catalogue NumberGF10
Brand Family Calbiochem®
SynonymsAnti-Transforming Growth Factor-α
References
ReferencesCoffey, R.J., et al. 1987. Nature 328, 817.
Derynck, R. 1986. J. Cell. Biochem. 32, 203.
Samsoondor, J., et al. 1986. J. Biol. Chem. 261, 14408.
Lee, D.C., et al. 1985. Molec. Cell. Biol. 5, 3644.
Kimball, E.S., et al. 1984. Cancer Res. 44, 3613.
Marquardt, H., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 4684.
Reynolds, F.H., Jr., et al. 1983. Nature 292, 259.
Todaro, G.J., et al. 1983. In Genes and Proteins in Oncogenesis, Vogel, H. and Weinstein, I.B. (Eds.) New York, Raven Press p. 165.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Positive controlA2058 cells or skin tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesOriginal Clone Sorvillo, J.M., et al. 1990. Oncogene 5, 377.
Key Applications Frozen Sections
Immunocytochemistry
Not Western Blot
Paraffin Sections
Application NotesFrozen Sections (1-5 µg/ml)
Immunocytochemistry (1-5 µg/ml)
Immunoblotting (not recommended)
Paraffin Sections (1-10 µg/ml, saponin pre-treatment required)
Application CommentsDoes not cross-react with human or mouse EGF. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant human TGF-α (~6 kDa mature form)
ImmunogenHuman
Clone213-4.4
HostMouse
IsotypeIgG2a
Species Reactivity
  • Canine
  • Hamster
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
GF10 0

Documentation

Anti-TGF-α (Ab-2) Mouse mAb (213-4.4) SDS

Title

Safety Data Sheet (SDS) 

Anti-TGF-α (Ab-2) Mouse mAb (213-4.4) Certificates of Analysis

TitleLot Number
GF10

References

Reference overview
Coffey, R.J., et al. 1987. Nature 328, 817.
Derynck, R. 1986. J. Cell. Biochem. 32, 203.
Samsoondor, J., et al. 1986. J. Biol. Chem. 261, 14408.
Lee, D.C., et al. 1985. Molec. Cell. Biol. 5, 3644.
Kimball, E.S., et al. 1984. Cancer Res. 44, 3613.
Marquardt, H., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 4684.
Reynolds, F.H., Jr., et al. 1983. Nature 292, 259.
Todaro, G.J., et al. 1983. In Genes and Proteins in Oncogenesis, Vogel, H. and Weinstein, I.B. (Eds.) New York, Raven Press p. 165.

Citations

Title
  • Mark R. Bush, et al. (1998) Evidence of Juxtacrine Signaling for Transforming Growth Factor a in Human Endometrium. 59, 1522-1529.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision23-August-2007 RFH
    SynonymsAnti-Transforming Growth Factor-α
    ApplicationFrozen Sections (1-5 µg/ml)
    Immunocytochemistry (1-5 µg/ml)
    Immunoblotting (not recommended)
    Paraffin Sections (1-10 µg/ml, saponin pre-treatment required)
    DescriptionPurified mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with P3 x 63 Ag 8.653 mouse myeloma cells (see application references). Recognizes the ~6 kDa TGF-α protein.
    BackgroundTransforming growth factor-alpha (TGF-α) is an acid- and heat-stable 50 amino acid protein of 5500 MW originally found in rodents and humans. TGF-α is 33% homologous at the amino acid level to epidermal growth factor (EGF). TGF-α binds to the EGF receptor, mediates tyrosine phosphorylation of the receptor, and promotes anchorage-independent growth of normal rat fibroblasts in soft agar in the presence of transforming growth factor-beta. TGF-α is secreted by a variety of transformed cells and tumors, embryonic cells, and some normal adult cells. TGF-α bioactivity has been found in the urine of cancer patients. It has been suggested that it may act as an autocrine growth factor for the induction or maintenance of malignancy.
    HostMouse
    Immunogen speciesHuman
    Immunogenrecombinant human TGF-α (~6 kDa mature form)
    Clone213-4.4
    IsotypeIgG2a
    Speciescanine, hamster, human, mouse, rat
    Positive controlA2058 cells or skin tissue
    FormLiquid
    FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
    Concentration Label Please refer to vial label for lot-specific concentration
    Preservative≤0.1% sodium azide
    CommentsDoes not cross-react with human or mouse EGF. Antibody should be titrated for optimal results in individual systems.
    Storage +2°C to +8°C
    Do Not Freeze Yes
    Toxicity Standard Handling
    ReferencesCoffey, R.J., et al. 1987. Nature 328, 817.
    Derynck, R. 1986. J. Cell. Biochem. 32, 203.
    Samsoondor, J., et al. 1986. J. Biol. Chem. 261, 14408.
    Lee, D.C., et al. 1985. Molec. Cell. Biol. 5, 3644.
    Kimball, E.S., et al. 1984. Cancer Res. 44, 3613.
    Marquardt, H., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 4684.
    Reynolds, F.H., Jr., et al. 1983. Nature 292, 259.
    Todaro, G.J., et al. 1983. In Genes and Proteins in Oncogenesis, Vogel, H. and Weinstein, I.B. (Eds.) New York, Raven Press p. 165.
    Citation
  • Mark R. Bush, et al. (1998) Evidence of Juxtacrine Signaling for Transforming Growth Factor a in Human Endometrium. 59, 1522-1529.
    		•
    Application referencesOriginal Clone Sorvillo, J.M., et al. 1990. Oncogene 5, 377.