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GF06 Anti-TGF-α (Ab-1) Mouse mAb (134A-2B3)

This Anti-TGF-α (Ab-1) Mouse mAb (134A-2B3) is validated for use in Immunoblotting for the detection of TGF-α (Ab-1).

Anti-TGF-α (Ab-1) Mouse mAb (134A-2B3) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Transforming Growth Factor-α

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Overview

Key Spec Table

Species ReactivityHostAntibody Type
H, RMMonoclonal Antibody
Description
OverviewRecognizes the ~6 kDa TGF-α protein in A2058 and ST-FeSVFRE cells.
Catalogue NumberGF06
Brand Family Calbiochem®
SynonymsAnti-Transforming Growth Factor-α
References
ReferencesSorvillo, J.M., McCormack, E.S. 1990. Oncogene 5, 377.
Coffey, R.J., et al. 1987. Nature 328, 817.
Derynck, R. 1986. J. Cell. Biochem. 32, 203.
Samsoondor, J., et al. 1986. J. Biol. Chem. 261, 14408.
Lee, D.C., et al. 1985 Mol. Cell. Biol. 5, 3644.
Kimball, E.S., et al. 1984. Cancer Res. 44, 3613.
Marquardt, H., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 4684.
Reynolds, F.H., Jr., et al. 1983. Nature 292, 259.
Todaro, G.J., et al. 1983. In Genes and Proteins in Oncogenesis, Vogel, H. and Weinstein, I.B. (Eds.) New York, Raven Press 165.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
Negative controlNIH3T3 cells
Positive controlA2058 or ST-FeSV FRE cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesOriginal Clone, Species Reactivity Sorvillo, J.M., McCormack, E.S., 1990. Oncogene 5, 377. Immunoblotting Leong, H., et al. 2001. Carcinogenesis 22, 1809. Bush, M.R., et al. 1998. Biol. Reprod. 59, 1522.
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1-5 µg/ml, see application references)
Application CommentsThis antibody shows no cross-reactivity with EGF. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant, human TGF-α (~6 kDa mature form)
ImmunogenHuman
Clone134A-2B3
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Special InstructionsWe recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) or aliquot and freeze (-20°C).
Global Trade Item Number
Catalogue Number GTIN
GF06 0