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MABS1753-25UG Anti-TC PTPase (PTPN2) Antibody, clone 9E9.1

Anti-TC PTPase (PTPN2), clone 9E9.1, Cat. No. MABS1753, is a mouse monoclonal antibody that detects T-cell protein-tyrosine phosphatase and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Research Category: Signaling Gene Symbol: PTPN2, PTPT UniProt Number: P17706
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MABS1753-25UG
25 μg  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1753-25UG
DescriptionAnti-TC PTPase (PTPN2) Antibody, clone 9E9.1
Alternate Names
  • Tyrosine-protein phosphatase non-receptor type 2
  • EC: 3.1.3.48
  • T-cell protein-tyrosine phosphatase
  • TCPTP
Background InformationTyrosine-protein phosphatase non-receptor type 2 (UniProt: P17706; also known as EC: 3.1.3.48, T-cell protein-tyrosine phosphatase, TCPTP) is encoded by the PTPN2 (also known as PTPT) gene (Gene ID: 5771) in human. TCPTP is a non-receptor type tyrosine-specific phosphatase that dephosphorylates a variety of receptor protein tyrosine kinases. It is also reported to dephosphorylate non-receptor tyrosine kinases, such as JAK 1, 2, 3, Src kinases, STAT1, 3, 5, 5B, and 6. TCPTP is ubiquitously expressed and two isoforms have been reported. Isoform 1 is expressed in T-cells and in placenta and is targeted to the endoplasmic reticulum by its C-terminal hydrophobic region. Isoform 2 is reported to be the major isoform that predominantly localizes to chromatin and is able to shuttle between the nucleus and the cytoplasm and to plasma membrane to dephosphorylate receptors. It doing so it negatively regulates numerous signaling pathways and biological processes, including hematopoiesis, inflammatory response, cell proliferation and differentiation, and glucose homeostasis. It is also reported to play a multifaceted and important role in the development of the immune system. In T-cell receptor signaling, it functions through dephosphorylation of FYN and LCK to control T-cell differentiation and activation. It also dephosphorylates CSF1R and thereby negatively regulates its downstream signaling and macrophage differentiation.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-TC PTPase (PTPN2), clone 9E9.1, Cat. No. MABS1753, is a mouse monoclonal antibody that detects T-cell protein-tyrosine phosphatase and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected TC PTPase (PTPN2) in human liver, human tonsil, and human cervical cancer tissues.
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected TC PTPase (PTPN2) in 10 µg of  Jurkat cell lysate.
Biological Information
ImmunogenGST/His-tagged recombinant fragment corresponding to 79 amino acids from the C-terminal half of human T-cell protein-tyrosine phosphatase.
Clone9E9.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 9E9.1 detects human T-cell protein-tyrosine phosphatase. It targets an epitope within 79 amino acids from the C-terminal half.
IsotypeIgG1
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • PTPN2
  • PTPT
Purification MethodProtein G purified
UniProt Number
Molecular Weight~48 KDa observed; 48.47 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in K562 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected TC PTPase (PTPN2) in 10 µg of K562 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS1753-25UG 04054839522208

Documentation