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MABS1913-100UG Anti-TAZ Antibody, clone 11

Anti-TAZ, clone 11, Cat. No. MABS1913, is a mouse monoclonal antibody that detects WW domain-containing transcription regulator protein 1 (TAZ) and has been tested for use in ELISA, Immunoprecipitation, and Western Blotting.

Anti-TAZ Antibody, clone 11 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: Wwtr1, Taz UniProt Number: Q9EPK5
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MABS1913-100UG
100 μg  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MELISA, IP, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1913-100UG
DescriptionAnti-TAZ Antibody, clone 11
Alternate Names
  • WW domain-containing transcription regulator protein 1
  • Transcriptional coactivator with PDZ-binding motif
Background InformationWW domain-containing transcription regulator protein 1 (UniProt: Q9EPK5; also known as Transcriptional coactivator with PDZ-binding motif, TAZ) is encoded by the Wwtr1 (also known as Taz) gene (Gene ID: 97064) in murine species. TAZ is an oncogenic transcriptional activator that acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. TAZ is highly expressed in kidney, heart, placenta, and lung tissues. Two isoforms of TAZ have been reported that are produced by alternative splicing. TAZ is concentrated along specific portions of the plasma membrane and also accumulates in punctate nuclear bodies. The ability of TAZ to regulate gene transcription is very tightly regulated through the phosphorylation-dependent binding of 14-3-3 protein. Upon phosphorylation TAZ is retained in cytoplasm by 14-3-3 zeta. Expression of NPHP9 is reported to inhibit the interaction of wild-type TAZ with 14-3-3 and NPHP9 is reported to abolish the phosphorylation of TAZ at Serine 89. TAZ is shown to be involved in non-small-cell lung cancer and its higher expression levels are observed in breast cancer and in breast cancer stem cells. It also regulates embryonic stem-cell self-renewal and promotes cell proliferation and epithelial-mesenchymal transition. TAZ binds to transcription factors via its WW domain, which is localized to amino acids 124-157. Its PZD-binding motif is located in amino acids 389-395 and this motif is essential for stimulated gene transcription.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-TAZ, clone 11, Cat. No. MABS1913, is a mouse monoclonal antibody that detects WW domain-containing transcription regulator protein 1 (TAZ) and has been tested for use in ELISA, Immunoprecipitation, and Western Blotting.
Key Applications
  • ELISA
  • Immunoprecipitation
  • Western Blotting
Application NotesWestern Blotting Analysis: 1 ug/mL from a representative lot detected TAZ in mouse lung tissue lysate.

Immunoprecipitation Analysis: A representative lot immunoprecipitated TAZ in Immunoprecipitation applications (Habbig, S., et. al. (2012). Hum Mol Genet. 21(26):5528-38).

Western Blotting Analysis: A representative lot detected TAZ in Western Blotting applications (Habbig, S., et. al. (2012). Hum Mol Genet. 21(26):5528-38).

ELISA Analysis: A representative lot detected TAZ in ELISA applications (Habbig, S., et. al. (2012). Hum Mol Genet. 21(26):5528-38).
Biological Information
ImmunogenHis-tagged Recombinant Protein corresponding to Mouse WW domain-containing transcription regulator protein 1 (TAZ).
Clone11
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 11 specifically detects WW domain-containing transcription regulator protein 1 (TAZ) in murine species.
IsotypeIgG2bκ
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Wwtr1
  • Taz
Purification MethodProtein G purified
UniProt Number
Molecular Weight~55 kDa observed; 43.62 and 49.83 kDa for isoform 1 and 2, respectively. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse kidney tissue lysate.

Western Blotting Analysis: 1 ug/mL of this antibody detected TAZ in mouse kidney tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS1913-100UG 04054839431838