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MABS2230-25UL Anti-Syntaxin 5 Antibody, clone 18C8

Anti-Syntaxin 5, clone 18C8, Cat. No. MABS2230, is a mouse monoclonal antibody that detects Syntaxin-5 and is tested for use in Immunocytochemistry, Immunoprecipitation, Inhibition Assay, and Western Blotting.

MABS2230-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABS2230-25UL
DescriptionAnti-Syntaxin 5 Antibody, clone 18C8
Alternate Names
  • STX-5
  • Sed5
Background InformationSyntaxin-5 (UniProt: Q08851; also known as STX-5) is encoded by the Stx5 (also known as Stx5a) gene (Gene ID: 65134) in rat. . Syntaxin-5 is a single-pass type IV membrane protein that localizes throughout the Golgi apparatus and is most abundant in the cis-most cisternae. It is found in the endoplasmic reticulum (ER)-Golgi intermediate compartment membrane. It has a cytoplasmic domain (aa 1-333), a transmembrane domain (aa 334-354), and a single amino acid vesicular domain (aa 355). Its expression has been reported in the brain, heart, spleen, lung, liver, kidney, and testis. It mediates ER to Golgi transport and together with vesicular transport factor p115 (USO1) and Golgi matrix protein GM130 (GOLGA2), it is involved in tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Syntaxin-5 is shown to protect cells against proteotoxicity and is involved in clearance of insoluble protein deposits. Clone 18C8 is shown to bind to syntaxin 5 SNARE motif and inhibit SNARE complex formation and prevents the SNARE motif from engaging other SNAREs. It binds to syntaxin 5 molecules with unengaged, SNARE motifs. (Ref.: Babazadeh, R., et al. (2019). Cell Rep. 28(8); 2096-2110; Diao, A., et al (2008); J. Biol. Chem. 283(11); 6957-6967; Williams, AL., et al. (2004). Mol. Biol. Cell. 15; 162-175).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
Quality LevelMQ200
Applications
ApplicationAnti-Syntaxin 5, clone 18C8, Cat. No. MABS2230, is a mouse monoclonal antibody that detects Syntaxin-5 and is tested for use in Immunocytochemistry, Immunoprecipitation, Inhibition Assay, and Western Blotting.
Key Applications
  • Western Blotting
  • Inhibits Activity/Function
  • Immunoprecipitation
  • Immunocytochemistry
Application NotesTested applications

Immunoprecipitation Analysis: A representative lot immunoprecipitated Syntaxin 5 in Immunoprecipitation applications (Williams, A.L., et al. (2004). Mol Biol Cell. 15(1):162-75).

Immunocytochemistry Analysis: A representative lot detected Syntaxin 5 in Immunocytochemistry applications (Williams, A.L., et al. (2004). Mol Biol Cell. 15(1):162-75).

Western Blotting Analysis: A representative lot detected Syntaxin 5 in Western Blotting applications (Williams, A.L., et al. (2004). Mol Biol Cell. 15(1):162-75).

Inhibition Assay: A representative lot inhibited the formation of a ternary SNARE complex. (Williams, A.L., et al. (2004). Mol Biol Cell.;15(1):162-75).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenGST-tagged recombinant fragment corresponding to 83 amino acids from the C-terminal half, SNARE motif of rat.
EpitopeC-terminal half
Clone18C8
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 18C8 is a is a mouse monoclonal antibody that detects Syntaxin-5. It targets an epitope within the cytoplasmic domain in the C-terminal half. It detects both isoform 1 and 2.
IsotypeIgG1κ
Species Reactivity
  • Rat
Species Reactivity NoteRat.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Stx5
Purification MethodProtein G purified
UniProt Number
Molecular Weight~42 and 34 kDa observed; 39.75 and 34.12 kDa, respectively for isoform 1 and 2. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Rat brain tissue extract and NRK cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Syntaxin-5 in Rat brain tissue extract and normal rat kidney epithelial (NRK) cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS2230-25UL 04065269126065