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MABS1241 Anti-Synembryn-A/Ric8A Antibody, clone 3E1

Detect Synembryn-A/Ric-8A using this mouse monoclonal Anti-Synembryn-A/Ric8A antibody, clone 3E1, Cat. No. MABS1241, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-Synembryn-A/Ric8A Antibody, clone 3E1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: Ric8a UniProt Number: Q80ZG1
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MABS1241
100 μg  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
R, HWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1241
DescriptionAnti-Synembryn-A/Ric8A Antibody, clone 3E1
Alternate Names
  • Protein Ric-8A
  • Resistance to inhibitors of cholinesterase 8
Background InformationSynembryn-A (UniProt: Q80ZG1; also known as Resistance to inhibitors of cholinesterase 8, Protein Ric-8A) is encoded by the Ric8a gene (Gene ID: 293614) in rat species. Resistance to inhibitors of cholinesterase 8 (RIC-8) is conserved in vertebrates and is concentrated in the cytoplasm of neurons. Mammalian Ric-8 proteins are G alpha subunit guanine nucleotide exchange factors. Ric-8A and Ric-8B collectively stimulate nucleotide exchange of all G alpha subunit classes by stabilizing the G alpha nucleotide-free transition state. Ric-8 is reported to directly participate in the post-translational folding of nascent G alpha subunits to promote G-protein biosynthesis. It may facilitate the transition of G alpha from a pre-folded globular state to its native state by promoting the first G alpha guanine nucleotide-binding event. Ric-8 interacts with GDP-bound G alpha proteins, but does not interact with G-alpha proteins when they are in complex with beta and gamma subunits. Overexpression of Ric-8 proteins in HEK293, NIH 3T3, or Sf9 cells is shown to enhance cellular G alpha levels. Embryonic stem cells lacking Ric-8A or Ric-8B produce defective G alpha subunits that turn over at about 10-fold higher rates than in wild-type cells. Ref.: Thomas, C.J. et al. (2011). PLoS ONE 6(8): e23197.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Synembryn-A/Ric-8A using this mouse monoclonal Anti-Synembryn-A/Ric8A antibody, clone 3E1, Cat. No. MABS1241, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected Synembryn-A/Ric-8A in human cerebral cortex, human cerebellum, and human testis tissues.

Western Blotting Analysis: A representative lot detected recombinant Synembryn-A/Ric-8A protein (Chan, P.Y., et al. (2013). Proc. Natl. Acad. Sci. USA. 110(10):3794-3799).
Biological Information
ImmunogenA full-length recombinant Ric-8A protein from rat.
Clone3E1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 3E1 detects Ric-8A in human brain.
IsotypeIgG2aκ
Species Reactivity
  • Rat
  • Human
Species Reactivity NoteHuman, Rat.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • Ric8a
Purification MethodProtein G purified
UniProt Number
Molecular Weight~60 kDa observed; 59.7 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Synembryn-A/Ric-8A in 10 µg of human brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS1241 04054839139154