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574597 Anti-Superoxide Dismutase (Cu/Zn Enzyme) Sheep pAb

This Anti-Superoxide Dismutase Sheep pAb is validated for use in WB, Immunoelectrophoresis, Frozen sections, Paraffin sections, Free-floating sections for the detection of Superoxide Dismutase.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-SOD

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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, Mk, M, RShPolyclonal Antibody
Description
OverviewRecognizes Cu/Zn SOD in red blood cells. Does not cross-react with Mn SOD.
Catalogue Number574597
Brand Family Calbiochem®
SynonymsAnti-SOD
References
ReferencesPasinelli, P., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 15763.
Product Information
FormLiquid
FormulationIn glycine-buffered saline, pH 7.4.
Positive controlHuman red blood cell lysate
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Free-floating Sections
Frozen Sections
Immunoblotting (Western Blotting)
Immunoelectrophoresis
Paraffin Sections
Application NotesImmunoblotting (1:1000)
Immunoelectrophoresis (see comments)
Frozen sections (see comments)
Paraffin sections (see comments)
Free-floating sections (see comments)
Application CommentsThis antibody is monospecific as determined by immunoelectrophoresis against human red blood cell lysate and confirmed by immunodiffusion against a human red blood cell lysate and a known anti-SOD (Cu/Zn) antibody. Does not exhibit cross-reactivity with SOD (Mn) by double diffusion or with human serum. This antibody is reported to be suitable for use with free-floating, frozen, or paraffin embedded sections when used in immunohistochemistry. Variables assocated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogenhighly purified, human erythrocyte SOD Cu/Zn.
ImmunogenHuman
HostSheep
IsotypeIgG
Species Reactivity
  • Human
  • Monkey
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
574597 0

Documentation

Anti-Superoxide Dismutase (Cu/Zn Enzyme) Sheep pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-Superoxide Dismutase (Cu/Zn Enzyme) Sheep pAb Certificates of Analysis

TitleLot Number
574597

References

Reference overview
Pasinelli, P., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 15763.

Citations

Title
  • Yu-Chin Lien, et al. (2006) Tumor necrosis factor receptor deficiency exacerbated Adriamycin-induced cardiomyocytes apoptosis: an insight into the Fas connection. Molecular Cancer Therapeutics 5, 261-269.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision16-August-2007 RFH
    SynonymsAnti-SOD
    ApplicationImmunoblotting (1:1000)
    Immunoelectrophoresis (see comments)
    Frozen sections (see comments)
    Paraffin sections (see comments)
    Free-floating sections (see comments)
    DescriptionSheep polyclonal antibody purified by DEAE ion-exchange chromatography. Recognizes the Cu/Zn superoxide dismutase protein.
    BackgroundSuperoxide dismutase Cu/Zn enzyme (SOD (Cu/Zn)) is a homodimeric metalloenzyme of aerobic organisms, comprised of subunits of ~16 kDa. Found in a variety of cell types, including erythrocytes, liver, and brain, it serves to protect cells against the oxidative damage of free radicals by catalyzing the conversion of superoxide anions (O2-) to molecular oxygen (O2) and hydrogen peroxide (H2O2).
    HostSheep
    Immunogen speciesHuman
    Immunogenhighly purified, human erythrocyte SOD Cu/Zn.
    IsotypeIgG
    Specieshuman, monkey, mouse, rat
    Positive controlHuman red blood cell lysate
    FormLiquid
    FormulationIn glycine-buffered saline, pH 7.4.
    Concentration Label Please refer to vial label for lot-specific concentration
    Preservative≤0.1% sodium azide
    CommentsThis antibody is monospecific as determined by immunoelectrophoresis against human red blood cell lysate and confirmed by immunodiffusion against a human red blood cell lysate and a known anti-SOD (Cu/Zn) antibody. Does not exhibit cross-reactivity with SOD (Mn) by double diffusion or with human serum. This antibody is reported to be suitable for use with free-floating, frozen, or paraffin embedded sections when used in immunohistochemistry. Variables assocated with assay conditions will dictate the proper working dilution.
    Storage Avoid freeze/thaw
    -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
    Toxicity Standard Handling
    ReferencesPasinelli, P., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 15763.
    Citation
  • Yu-Chin Lien, et al. (2006) Tumor necrosis factor receptor deficiency exacerbated Adriamycin-induced cardiomyocytes apoptosis: an insight into the Fas connection. Molecular Cancer Therapeutics 5, 261-269.
    		•