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AB5603-25UG Anti-Sox2 (rabbit polyclonal)

Anti-Sox2 Antibody is an antibody against Sox2 for use in IC & WB with more than 60 product citations.

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AB5603-25UG
25 μg  
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Description
Catalogue NumberAB5603-25UG
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Sox2 (rabbit polyclonal)
Alternate Names
  • Transcription factor SOX-2
  • Sex determining region Y-box 2
  • SRY-related HMG-box gene 2
  • Transcription factor SOX2
Background InformationTranscription factor SOX-2 (UniProt P48431; also known as Sex determining region Y-box 2, SRY-related HMG-box gene 2, Transcription factor SOX2) is encoded by the SOX2 (also known as ANOP3, MCOPS3) gene (Gene ID 6657) in human. Sox family of genes are related to the mammalian sex determining gene SRY. These genes contain sequences coding the HMG-box domains that mediate sequence-specific DNA binding. Sox genes encode transcriptional regulators involved in cell fate decision during development and the control of diverse developmental processes. The highly complex group of SOX genes cluster at at least 40 genomic loci and at least 30 SOX genes have been identified. Sox2 plays an essential role in maintaining the pluripotency and self-renewal of undifferentiated embryonic stem cells.
References
Product Information
FormatAffinity Purified
Control
  • Immunoblot: Mouse or human embryonic stem cell lysate, mouse embryonic germ cell lysate
    Immunocytochemistry: Human embryonic stem cells (H9 line)
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Sox2 Antibody is an antibody against Sox2 for use in IC & WB with more than 60 product citations.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunofluorescence
  • Immunohistochemistry
Application NotesImmunocytochemistry Analysis: 10 µg/mL from a representative lot detected Sox2 in H9 human stem cells.
Immunocytochemistry Analysis: A representative lot detected nuclear Sox2 immunoreactivity in 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized human embryonic stem cells (hESCs) by fluorescent immunocytochemistry (Kingham, E., et al. (2013). Small. 9(12):2140-2151).
Immunocytochemistry Analysis: A representative lot detected Sox2 immunoreactivity by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed iPSCs generated from human kidney tubular renal epithelial cells (Montserrat, N., et al. (2012). J. Biol. Chem. 287(29):24131-24138).
Immunocytochemistry Analysis: A representative lot detected Sox2 immunoreactivity by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.5% Triton X-100-permeabilized iPSCs generated from the skin cells of patients with Parkinson's disease (Sánchez-Danés, A., et al. (2012). EMBO Mol. Med. 4(5):380-395).
Immunocytochemistry Analysis: Representative lots detected Sox2 immunoreactivity by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.2% Triton X-100-permeabilized iPSCs generated from mouse ear cells and MEF (Burns, J.C., et al. (2012). PLoS One. 7(10):e48704; Hadadeh, O., et al. (2012). PLoS One. 7(11):e49065).
Western Blotting Analysis: A representative lot detected Sox2 in mouse embryonic stem cell (mESC) nuclear extracts (Banaszynski, L.A., et al. (2013). Cell. 155(1):107-120).
Immunofluorescence Analysis: A representative lot detected Sox2-positive cells in 4% paraformaldehyde-fixed, 0.3% Triton X-100-permeabilized mouse brain vibratome sections by fluorescent immunohistochemistry (Matsuda, S., et al. (2012). J. Neurosci. 32(36):12543-12557).
Immunohistochemistry Analysis: A representative lot detected Sox2 immunoreactivity in 4% paraformaldehyde-fixed whole mount mouse embryo sections (van Rooijen, C., et al. Development. 139(14):2576-2583).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to a C-terminal region sequence of human Sox2.
EpitopeC-terminal region.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis antibody recognizes Sox2 C-terminal region.
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse. Predicted to react with Rat, Bovine, Rhesus Macaque based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • SOX2
  • ANOP3
  • MCOPS3
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~39 kDa observed. 34.31 kDa (human) and 34.45 (mouse) calculated. Uncharacterized band(s) may appear in some lysates
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mESC lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Sox2 in 10 µg of mESC lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB5603-25UG 04054839342578

Documentation

Anti-Sox2 (rabbit polyclonal) Certificates of Analysis

TitleLot Number
Anti-Sox2 (rabbit polyclonal) - 3328180 3328180
Anti-Sox2 (rabbit polyclonal) - 3517510 3517510
Anti-Sox2 (rabbit polyclonal) - 3715668 3715668
Anti-Sox2 (rabbit polyclonal) - 3753313 3753313
Anti-Sox2 (rabbit polyclonal) - 3780596 3780596
Anti-Sox2 (rabbit polyclonal) - 3821565 3821565
Anti-Sox2 (rabbit polyclonal) - 3849498 3849498
Anti-Sox2 (rabbit polyclonal) - 3866801 3866801
Anti-Sox2 (rabbit polyclonal) - 3883587 3883587
Anti-Sox2 (rabbit polyclonal) - 3911801 3911801
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