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ABN1475 Anti-Serotonin receptor 3A/HTR3A

Anti-Serotonin receptor 3A/HTR3A Antibody, Cat. No. ABN1475, is a highly specific rabbit polyclonal antibody that targets HTR3A and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-Serotonin receptor 3A/HTR3A MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: HTR3A, 5HT3R, HTR3 UniProt Number: P46098
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ABN1475
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABN1475
DescriptionAnti-Serotonin receptor 3A/HTR3A
Alternate Names
  • 5-hydroxytryptamine receptor 3A
  • 5-HT-3
  • 5-HT3-A
  • 5-HT3A
  • 5-HT3R
  • 5-hydroxytryptamine receptor 3
  • Serotonin receptor 3A
  • Serotonin-gated ion channel receptor
Background Information5-hydroxytryptamine receptor 3A (UniProt P46098; also known as 5-HT-3, 5-HT3-A, 5-HT3A, 5-HT3R, 5-hydroxytryptamine receptor 3, Serotonin receptor 3A, Serotonin-gated ion channel receptor) is encoded by the HTR3A (also known as 5HT3R, HTR3) gene (Gene ID 3359) in human. Hydroxytryptamine (5-HT) is synthesized in enterochromaffin cells in the intestine and in serotonergic nerve terminals. In the periphery, 5-HT mediates gastrointestinal motility, platelet aggregation, and contraction of blood vessels. Many functions of the central nervous system are influenced by 5-HT, including sleep, motor activity, sensory perception, arousal and appetite. A family of 12 GPCRs and one ion channel mediate the biological effects of 5-HT. 5-hydroxytryptamine receptor 3 (5-HT3R) is a ligand-gated ion channel involved in many neurophysiological functions and is widely expressed throughout the brain and in peripheral organs. Five different subunits (A through E) have been identified, with the homopentameric 5-HT3A being the most studied form. 5-HT3A is produced with a signal peptide sequence (a.a. 1-23), which is removed posttranslationally to yield the mature 4-transmembrane (a.a. 242-268, 274-292, 303-321, 456-475) ion channel with both its N- and C-terminal ends exposed extracellularly (a.a. 24-241 and 476-478).
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide
Quality LevelMQ100
Applications
ApplicationAnti-Serotonin receptor 3A/HTR3A Antibody, Cat. No. ABN1475, is a highly specific rabbit polyclonal antibody that targets HTR3A and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected serotonin receptor 3A/HTR3A in human prostate cancer, as well as in normal cerebral cortex, heart and tonsil tissue sections.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to a sequence from the second cytomplasmic domain of human Serotonin receptor 3A/HTR3A.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects human Serotonin receptor 3A/HTR3A by targeting a sequence within the second cytoplasmic domain.
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • HTR3A
  • 5HT3R
  • HTR3
Purification MethodAffinity purified.
UniProt Number
Molecular Weight~55 kDa observed. 52,79/55.28 kDa (mature/pro-isoform 1; 5-HT3R-AS), 55.90/58.39 kDa (mature/pro-isoform 2; 5-HT3R-AL), 52.79/53.76 kDa (mature/pro-isoform 3), 52.79/55.89 kDa (mature/pro-isoform 4), 55.90/59.00 kDa (mature/pro-isoform 5). Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human cerebral cortex tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected serotonin receptor 3A/HTR3A in 10 µg of human cerebral cortex tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABN1475 04054839049767