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05-615-I-25UL Anti-SUV39H1 Antibody, clone MG44

Anti-SUV39H1, clone MG44, Cat. No. 05-615-I, is a mouse monoclonal antibody that detects Histone-lysine N-methyltransferase SUV39H1 and is tested for use in Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, and Western Blotting.

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05-615-I-25UL
25 µL  
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Overview

Replacement Information
Description
Catalogue Number05-615-I-25UL
DescriptionAnti-SUV39H1 Antibody, clone MG44
Alternate Names
  • Histone-lysine N-methyltransferase SUV39H1
  • EC:2.1.1.355
  • Histone H3-K9 methyltransferase 1
  • H3-K9-HMTase 1
  • Lysine N-methyltransferase 1A
  • Position-effect variegation 3-9 homolog
  • Suppressor of variegation 3-9 homolog 1
  • Su(var)3-9 homolog 1
Background InformationHistone-lysine N-methyltransferase SUV39H1 (UniProt: O43463; also known as EC:2.1.1.355, Histone H3-K9 methyltransferase 1, H3-K9-HMTase 1, Lysine N-methyltransferase 1A, Position-effect variegation 3-9 homolog, Suppressor of variegation 3-9 homolog 1, Su(var)3-9 homolog 1, SUV39H1) is encoded by the SUV39H1 (also known as KMT1A, SUV39H) gene (Gene ID: 6839) in human. SUV39H1 specifically trimethylates lysine 9 of histone H3 using monomethylated H3 lysine 9 as the substrate. It is also reported to weakly methylate histone H1 in vitro. Trimethylation of histone H3 at lysine 9 represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. SUV39H1 mainly functions in heterochromatin regions and plays a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. SUV39H1 is shown to be targeted to histone H3 via its interaction with RB1 and is involved in several key processes, such as repression of MYOD1-stimulated differentiation, regulation of the control switch for exiting the cell cycle and entering differentiation, and regulation of telomere length. It can undergo phosphorylation at serine residues, and to a lesser degree, on threonine residues and the phosphorylated form is stabilized by SBF1 and is less active in its transcriptional repressor function. SUV39H1 contains a SET domain (aa 243-366) that contains the active site, however, both the pre-SET (aa 179-240) and post-SET 9 (aa 396-412) domains are required for the methyltransferase activity.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-SUV39H1, clone MG44, Cat. No. 05-615-I, is a mouse monoclonal antibody that detects Histone-lysine N-methyltransferase SUV39H1 and is tested for use in Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, and Western Blotting.
Key Applications
  • Chromatin Immunoprecipitation (ChIP)
  • Immunoprecipitation
  • Western Blotting
Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected SUV39H1 in U2OS and COS-7 cell lysates.

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected SUV39H1 in Chromatin Immunoprecipitation applications (Giuseppina, P., et. al. (2017). Nat. Commun. 8:15622).

Immunoprecipitation Analysis: A representative lot immunoprecipitated SUV39H1 in Immunoprecipitation applications (Giuseppina, P., et. al. (2017). Nat. Commun. 8:15622).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenMBP-tagged full-length recombinant human Histone-lysine N-methyltransferase SUV39H1.
Epitopeunknown
CloneMG44
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone MG44 is a mouse monoclonal antibody that detects Histone-lysine N-methyltransferase SUV39H1.
IsotypeIgG
Species Reactivity
  • Monkey
  • Human
Species Reactivity NoteMonkey, Human. Predicted to react with Mouse based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • SUV39H1
  • KMT1A
  • SUV39H
Purification MethodProtein G purified
UniProt Number
Molecular Weight~48 kDa observed; 47.91 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HCT116 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SUV39H1 in HCT116 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
05-615-I-25UL 04061841878308

Documentation

Anti-SUV39H1 Antibody, clone MG44 SDS

Title

Safety Data Sheet (SDS) 

Anti-SUV39H1 Antibody, clone MG44 Certificates of Analysis

TitleLot Number
Anti-SUV39H1, clone MG44 - Q3517675 Q3517675

References

Reference overviewPub Med ID
A promoter-proximal transcript targeted by genetic polymorphism controls E-cadherin silencing in human cancers
Giuseppina Pisignano 1 , Sara Napoli 1 , Marco Magistri 1 , Sarah N Mapelli 1 , Chiara Pastori 1 , Stefano Di Marco 1 , Gianluca Civenni 1 , Domenico Albino 1 , Claudia Enriquez 1 , Sara Allegrini 1 , Abhishek Mitra 1 , Gioacchino D'Ambrosio 2 , Maurizia Mello-Grand 3 , Giovanna Chiorino 3 , Ramon Garcia-Escudero 1 4 , Gabriele Varani 5 , Giuseppina M Carbone 1 , Carlo V Catapano
Nat Commun  8  15622  2017

Show Abstract 		28555645 28555645