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MABS2312-25UL Anti-STOM Antibody, clone GARP-50

Anti-STOM, clone GARP-50, Cat. No. MABS2312, is a mouse monoclonal antibody that detects STOM and is tested for use in Electron Microscopy, Immunoaffinity Purification, Immunofluorescence, Immunoprecipitation, and Western Blotting.

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MABS2312-25UL
25 µL  
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Overview

Replacement Information
Description
Catalogue NumberMABS2312-25UL
DescriptionAnti-STOM Antibody, clone GARP-50
Alternate Names
  • Erythrocyte band 7 integral membrane protein
  • Erythrocyte membrane protein band 7.2
  • Protein 7.2b
  • Stomatin
Background InformationStomatin (UniProt: P27105; also known as Erythrocyte band 7 integral membrane protein, Erythrocyte membrane protein band 7.2, Protein 7.2b) is encoded by the STOM (also known as BND7, EPB72) gene (Gene ID: 2040) in human. Stomatin is a major integral protein of erythrocyte membranes that is palmitoylated and forms homo-oligomers and associates with lipid rafts. It contains a cytoplasmic domain (aa 1-25); an intramembrane domain (aa 26-54); and a cytoplasmic domain (aa 55-288). Its amino acid residues 265-273 are essential for homooligomerization. Stomatin was named after the rare human haemolytic anaemia hereditary stomatocytosis. It is ubiquitously expressed in all tissues and higher expression is observed in hematopoietic cells. Lower expression is observed in the brain. It is known to associate with the plasma membrane and cytoplasmic vesicles of fibroblasts, epithelial and endothelial cells, and specialized endosomes/granules in hematopoietic cells. At its N-terminal region it contains a 24-residue, basic domain that is phosphorylated at serine 10 by a cAMP-dependent protein kinase. This is followed by a 29-residue hydrophobic domain that is associated with the cytoplasmic face of the membrane and is palmitoylated at Cys-30. This hydrophobic intramembrane domain (residues 26 54) contains the conserved residue Pro-47, which is essential for its rare monotopic membrane protein structure. Adjacent to the intramembrane domain is a short sequence, KIIKEYERAII (aa 55-65) that displays similarity to the cholesterol-binding CRAC (cholesterol recognition/interaction amino acid consensus) motif. In its structure palmitoylated Cys-87 is essential for correct membrane attachment. Stomatin is known to interact with various ion channels and modulates their activity. It is also reported to inhibit the activity of Akt, suppress cancer cell proliferation, enhance apoptosis in vitro and inhibit xenograft tumor growth in vivo.Two isoforms of stomatin have been described that are produced by alternative splicing. (Ref.: Rungaldier, S., et al. (2017). PLoS One. 12(6):e0178646; Stewart, GW. (1997). Int. J. Biochem. Cell Biol. 29(2); 271-274; Christine M. Hiebi-Dirschmied, CM., et al. (1991). Biochim. Biophys. Acta. 1065; 195-202).
References
Product Information
FormatPurified
Control
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PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-STOM, clone GARP-50, Cat. No. MABS2312, is a mouse monoclonal antibody that detects STOM and is tested for use in Electron Microscopy, Immunoaffinity Purification, Immunofluorescence, Immunoprecipitation, and Western Blotting.
Key Applications
  • Electron Microscopy
  • Immunoaffinity Purification
  • Immunofluorescence
  • Immunoprecipitation
  • Western Blotting
Application NotesTested applications

Electron Microscopy: A representative lot detected STOM in Immunoelectron Microscopy application (Feuk-Lagerstedt, E., et al. (2002). J Leukoc Biol. 72(5):970-7).

Immunoprecipitation Analysis: A representative lot immunoprecipitated STOM in Immunoprecipitation application (Snyers, L., et al. (1998). J Biol Chem. 273(27):17221-6; Snyers, L., et al. (1999). FEBS Lett. 449(2-3):101-4).

Immunoaffinity Purification: A representative lot was used to isolate STOM by immunoaffinity chromatography..(Hiebl-Dirshmied, C.M., et al. (1991). Biochim Biophys Acta. 1065(2):195-202; Salzer, U., et al. (1993). Biochim Biophys Acta. 1151(2):149-52).

Immunofluorescence Analysis: A representative lot detected STOM in Immunofluorescence application (Snyers, L., et al. (1997). Eur J Cell Biol. 73(3):281-5; Snyers, L., et al. (1999). Eur J Cell Biol. 78(11):802-12).

Western Blotting Analysis:

Western Blotting Analysis: A representative lot detected STOM in Western Blotting application (Hiebl-Dirshmied, C.M., et al. (1991). Biochim Biophys Acta. 1065(2):195-202; Snyers, L., et al. (1998). J Biol Chem. 273(27):17221-6; Snyers, L., et al. (1999). Eur J Cell Biol. 78(11):802-12; Snyers, L., et al. (1999). FEBS Lett. 449(2-3):101-4; Salzer, U., et al. (2001). Blood. 97(4):1141-3; Mairhofer, M., et al. (2002). Blood. 100(3):897-904; Feuk-Lagerstedt, E., et al. (2002). J Leukoc Biol. 72(5):970-7).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenPurified native Stomatin from human erythrocytes.
EpitopeN-terminal
CloneGARP-50
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone GARP-50 is a mouse monoclonal antibody that detects Stomatin (STOM). It targets an epitope within the N-terminal region.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Canine, Bovine, Horse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • STOM
Purification MethodProtein G purified
UniProt Number
Molecular Weight~32 kDa observed; 31.73 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysates.

Western Blotting Analysis: A 1:500 dilution of this antibody detected STOM in HeLa cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS2312-25UL 04065269049166