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MABF3074-100UG Anti-SRF Antibody, clone 2C5

Anti-SRF, clone 2C5, Cat. No. MABF3074, is a rat monoclonal antibody that detects serum response factor (SRF) and is used in Chromatin Immunoprecipitation, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

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MABF3074-100UG
100 μg  
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Overview

Replacement Information
Description
Catalogue NumberMABF3074-100UG
DescriptionAnti-SRF Antibody, clone 2C5
Alternate Names
  • Serum response factor
Background InformationSerum response factor (UniProt: Q9JM73; also known as SRF) is encoded by the Srf gene (Gene ID: 20807) in murine species. SRF is a transcription factor that binds to a serum response element (SRE) associated with a variety of genes, including immediate early genes such as c-Fos, FosB, JunB, Egr-1 and -2; neuronal genes such as nurr1 and nur77. It binds to DNA as a dimer and its DNA binding region is localized in amino acids 129-218. By regulating the expression of several genes, it controls cell growth and differentiation, neuronal transmission, and muscle development. Together with Myocardin Related Transcription Factor A (MRTFA) transcription coactivator, it controls the expression of genes regulating cytoskeleton development, morphogenesis, and cell migration. It is also required for cardiac differentiation and maturation. SRF is shown to be activated by a variety of agents, including serum, lysophosphatidic acid, lipopolysaccharide, 12-O-tetradecanoylphorbol-13-acetate (TPA), and cytokines. Keratinocytes in normal human and mouse skin are reported to express high levels of SRF, but its expression is strongly downregulated in the hyperproliferative epidermis of wounded and psoriatic skin. Loss of SRF is known to disrupt epidermal homeostasis. Mice lacking SRF in cardiac tissue display lethal cardiac defects between E10.5 and E13.5. (Ref.: Hu, X., et al. (2019). Nat. Commun. 10(1); 1695; Koegel, H., et al. (2009). J. Clin. Invest. 119(4); 899-910; Parlakian , A., et al (2004). Mol. Cell Biol. 24(12); 5281-5289; Chai, J., and Tarnawski, AS. (2002). J. Physiol. Pharmacol. 53(2); 147-157).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-SRF, clone 2C5, Cat. No. MABF3074, is a rat monoclonal antibody that detects serum response factor (SRF) and is used in Chromatin Immunoprecipitation, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
Key Applications
  • Chromatin Immunoprecipitation (ChIP)
  • Immunofluorescence
  • Immunohistochemistry (Paraffin)
  • Immunoprecipitation
  • Western Blotting
Application NotesTested Applications

Immunofluorescence Analysis: A representative lot detected SRF in Immunofluorescence applications (Koegel, H., et al. (2009). J Clin Invest. 119(4):899-910).

Immunohistochemistry Applications: A representative lot detected SRF in Immunohistochemistry applications (Koegel, H., et al. (2009). J Clin Invest. 119(4):899-910).

Western Blotting Analysis: A representative lot detected SRF in Western Blotting applications (Koegel, H., et al. (2009). J Clin Invest. 119(4):899-910).

Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected SRF in Chromatin Immunoprecipitation applications (Hu, X., et al. (2019). Nat Commun. 10(1):1695).

Immunoprecipitation Analysis: A representative lot detected SRF in Immunoprecipitation applications (Hu, X., et al. (2019). Nat Commun. 10(1):1695).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenBacterially overexpressed His-tagged murine Serum response factor.
EpitopeUnknown
Clone2C5
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRat
SpecificityClone 2C5 is a rat monoclonal antibody that detects murine Serum response factor (SRF).
IsotypeIgG2aκ
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Srf
Purification MethodProtein G purified
UniProt Number
Molecular Weight51.25 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human psoriatic skin tissue sections.

Immunohistochemistry (Paraffin) Analysis (IHC(P)): A 1:50 dilution of this antibody detected Serum response factor (SRF) in human psoriatic skin tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF3074-100UG 04065268041994