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MABC683 Anti-SMAD6 Antibody, clone 5H3

Detect Smad using this mouse monoclonal antibody, Anti-SMAD6 Antibody & clone 5H3 validated for use in western blotting.

Anti-SMAD6 Antibody, clone 5H3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: MADH6, SMAD6 UniProt Number: O43541
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MABC683
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBMAscitesMonoclonal Antibody
Description
Catalogue NumberMABC683
DescriptionAnti-SMAD6 Antibody, clone 5H3
Alternate Names
  • Mothers against decapentaplegic homolog 6
  • MAD homolog 6
  • Mothers against DPP homolog 6
  • SMAD family member 6
  • SMAD 6
  • Smad6
  • hSMAD6
Background InformationSMAD6 is the gene name for a protein that is a homolog of the fly gene Mothers against decapentaplegic, and in humans it is called Mothers against decapentaplegic homolog 6 (MAD homolog 6). Other names include SMAD family member 6 and Mothers against DPP homolog 6 and another gene name of MADH6. By whatever name, the protein is a transcription factor that binds to regulatory elements within the nucleus and mediates transcription. The effect is to act as a mediator of TGF-beta and BMP mediated anti-inflammatory signaling activity. SMAD6 suppresses IL1R-TLR signaling, thus preventing NF-kappa B activation and activation of proinflammatory genes. SMAD6 interacts with numerous other intracellular proteins including NEDD4L, STAMBP, PRKK, RNF111, and AXIN1. SMAD6 is expressed in a wide variety of tissues with higher levels being seen in lung and cardiac tissues. Recently SMAD6 has been shown to promote neuronal differentiation by inhibiting the Wnt/beta catenin signaling pathway in addition to inhibiting the BMP signaling. Finally in cancer SMAD6 via its suppression of TGF-beta induced growth inhibition can potentiate the transformed phenotype. Moreover SMAD6 is often over expressed in many cancers, which suppresses G0-G1 arrest and apoptosis which might otherwise normally occur, thus leaving SMAD6 playing a critical role in supporting cancer cell growth and survival. EMD-Millipore’s Anti-SMAD6 mouse monoclonal antibody has been tested in western blot against human A431, HeLa, and Jurkat cell lysates.
References
Product Information
FormatAscites
Control
  • A431, HeLa, and Jurkat cell lysates
PresentationMouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Smad using this mouse monoclonal antibody, Anti-SMAD6 Antibody & clone 5H3 validated for use in western blotting.
Key Applications
  • Western Blotting
Biological Information
ImmunogenPurified recombinant fragment of human SMAD6 expressed in E. Coli.
Clone5H3
HostMouse
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Gene Symbol
  • MADH6
  • SMAD6
Purification MethodUnpurified
UniProt Number
Molecular Weight~55 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in A431, HeLa, and Jurkat cell lysates.

Western Blot Analysis: A 1:500-2000 dilution of this antibody detected SMAD6 in A431, HeLa, and Jurkat cell lysates.

Optimal working dilutions must be determined by end user.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABC683 04053252914508