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AP1134 Anti-SET Mouse mAb (M1-F5)

Anti-SET Mouse mAb (M1-F5) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-HLA-DR- Associated Protein II

AP1134
  
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Overview

Replacement Information

Key Spec Table

Host
M
Description
Overview

This product has been discontinued.





Recognizes the ~56-58 kDa SET protein.
Catalogue NumberAP1134
Brand Family Calbiochem®
SynonymsAnti-HLA-DR- Associated Protein II
Application Data
Detection of human SET by immunoblotting. Sample: Recombinant human SET. Primary antibody: Anti-SET Mouse mAb (M1-F5) (Cat. No. AP1134) (1-5 µg/ml). Detection: chemiluminescence.
References
Product Information
Formliquid
FormulationIn PBS, pH 7.2.
PreservativeNone
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended starting concentration is 10 µg/ml, 1 µg/well, 100 µl total volume per well. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenFull-length, recombinant human SET (aa1-278) expressed as a GST fusion protein
ImmunogenNA
CloneM1-F5
HostMouse
IsotypeIgG1
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AP1134 0

Documentation

Anti-SET Mouse mAb (M1-F5) Certificates of Analysis

TitleLot Number
AP1134
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision20-October-2010 JLN
SynonymsAnti-HLA-DR- Associated Protein II
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application Data
Detection of human SET by immunoblotting. Sample: Recombinant human SET. Primary antibody: Anti-SET Mouse mAb (M1-F5) (Cat. No. AP1134) (1-5 µg/ml). Detection: chemiluminescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~56-58 kDa SET protein.
BackgroundSET is a multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone binding. There are two known isoforms, both of which serve as potent inhibitors of protein phosphatase 2A. Furthermore, both isoforms inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. Unlike isoform 1 characteristics, isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition.
HostMouse
Immunogen speciesNA
ImmunogenFull-length, recombinant human SET (aa1-278) expressed as a GST fusion protein
CloneM1-F5
IsotypeIgG1
Specieshuman
Formliquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended starting concentration is 10 µg/ml, 1 µg/well, 100 µl total volume per well. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review