Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABF3072-100UG Anti-SARS-CoV RBD Antibody, clone m396

Anti-SARS-CoV RBD, clone m396, Cat. No. MABF3072, is a human recombinant monoclonal antibody that detects Spike protein of SARS-CoV and is tested for use in ELISA, Neutralizing, and Western Blotting.

View Products on Sigmaaldrich.com
MABF3072-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABF3072-100UG
DescriptionAnti-SARS-CoV RBD Antibody, clone m396
Alternate Names
  • Spike glycoprotein
  • S glycoprotein
  • E2
  • Peplomer protein
Background InformationSpike glycoprotein (UniProt: P59594; also known as S glycoprotein, E2, Peplomer protein) is encoded by the S (also known as 2) gene (Gene ID: 1489668) in SARS-CoV-2 virus. The SARS-CoV-2 is a positive-strand RNA virus that causes severe respiratory syndrome in human. The mature SARS-CoV-2 contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S). E and M proteins help in viral assembly and N protein is needed for RNA synthesis. The S protein is a single-pass type I, homotrimeric, membrane glycoprotein that is responsible for virus binding and entry into host cell. It is synthesized with a signal peptide (aa 1-12), which is subsequently cleaved off to generate the mature protein that contains an extracellular domain (aa 13-1213), a transmembrane domain (aa 1214-1234), and a cytoplasmic domain 1235-1273). The S protein is further processed into S1 (aa 13-685) and S2 (aa 686-1273) subunits by host cell furin. The presence of a furin polybasic cleavage site in S protein from SARS-CoV-2 sets it apart from S protein in SARS-CoV that possesses a monobasic S1/S2 cleavage site. The S1 subunit has the receptor binding domain (RBD; aa 319-541) that mediates entry of SARS-CoV-2 into sensitive cells through the peptidase domain of host Angiotensin-converting enzyme 2 (ACE2) with high affinity (KD = 15 nM). The S2 protein, which is reported to be well conserved, is responsible for membrane fusion. Clone m396 is shown to neutralize SARS-CoV-2 virus by disrupting ACE2/RBD binding. It is also effective in neutralizing both SARS-CoV-1 and SARS-CoV-2 pseudoparticles. (Ref.: Ju, B., et al. (2020). Nature. 584(7819); 115-119; Cao, Y., et al. (2020). Cell. 182(1); 73-84).
References
Product Information
FormatPurified
PresentationPurified human recombinant monoclonal antibody IgG1 in PBS without azide.
Quality LevelMQ200
Applications
ApplicationAnti-SARS-CoV RBD, clone m396, Cat. No. MABF3072, is a human recombinant monoclonal antibody that detects Spike protein of SARS-CoV and is tested for use in ELISA, Neutralizing, and Western Blotting.
Key Applications
  • Western Blotting
  • Neutralizing
  • ELISA
Application NotesTested Applications

Neutralizing: A representative lot neutralized SARS-CoV in Neutralizing applications (Zhu, Z., et al. (2007). Proc Natl Acad Sci USA.;104(29):12123-8).

ELISA Analysis: Various dilutions of this antibody detected His-tagged SARS-CoV Receptor Binding Domain (RBD) of SARS-CoV Spike protein.

ELISA Analysis: A representative lot detected SARS-CoV RBD in ELISA applications (Ju, B., et al. (2020). Nature.;584(7819):115-119).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenFrom human IgG phage library panned against the receptor-binding domain (RBD) of the SARS-CoV spike protein.
EpitopeExtracellular domain
Clonem396
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostHuman Recombinant
SpecificityClone m396 is a human recombinant monoclonal antibody that detects SARS-CoV spike protein. It targets an epitope within the receptor binding domain.
IsotypeIgG1
Species Reactivity
  • Virus
Species Reactivity NoteSARS-CoV
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • S
Purification MethodProtein A purified
UniProt Number
Molecular Weight~34 kDa observed for recombinant His-tagged receptor binding domain; 139.13 kDa calculated for spike protein. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting with His-tagged recombinant receptor binding domain of SARS-CoV spike protein.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected recombinant receptor binding domain of SARS-CoV spike protein.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF3072-100UG 04065266791334