Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

04-959 Anti-Retinoid-Related Orphan Receptor γ T Antibody, clone RORg2

Detect Retinoid-Related Orphan Receptor γ T using this Anti-Retinoid-Related Orphan Receptor γ T Antibody, clone RORg2 validated for use in WB, FC & IF.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Inflammation & Autoimmune Mechanisms Gene Symbol: RORG, RZRG, NR1F3, RORC, TOR UniProt Number: P51450
View Products on Sigmaaldrich.com
04-959
100 µg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MWBAHmPurifiedMonoclonal Antibody
Description
Catalogue Number04-959
DescriptionAnti-Retinoid-Related Orphan Receptor γ T Antibody, clone RORg2
Alternate Names
  • RAR-related orphan receptor C
  • RAR-related orphan receptor gamma
  • retinoic acid-binding receptor gamma
  • nuclear receptor ROR-gamma
  • retinoid-related orphan receptor gamma
  • Nuclear receptor RZR-gamma
  • RZR-GAMMA
  • Nuclear receptor subfamily 1 group F member 3
  • RAR-related orphan receptor C, isoform a
  • Retinoid-related orphan receptor-gamma
Background InformationRetinoid-related orphan receptor-gamma t (RORγT) is one of two RORγ isoforms belonging to the nuclear hormone receptor family and contains a single nuclear receptor DNA-binding domain. RORγT is a negative regulator of TNFDF6/FASL expression and T-cell production of IL2. The upregulation of RORγT is a requirement for TH17 cell differentiation. Inhibition of RORγT is induced by TGF-β’s interaction with Foxp3. Expression is observed mainly in immature thymocytes, and, during fetal development, is found exclusively in lymphoid-inducer (LTi) cells.
References
Product Information
FormatPurified
Control
  • EL4 cell lysate
PresentationPurified hamster monoclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Retinoid-Related Orphan Receptor γ T using this Anti-Retinoid-Related Orphan Receptor γ T Antibody, clone RORg2 validated for use in WB, FC & IF.
Key Applications
  • Western Blotting
Biological Information
ImmunogenHistidine-tagged recombinant protein corresponding to mouse Retinoid-Related Orphan Receptor γ T (RORγT).
EpitopeUnknown
CloneRORg2
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostArmenian Hamster
Species Reactivity
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • RORG
  • RZRG
  • NR1F3
  • RORC
  • TOR
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: Orphan nuclear receptor. Isoform 2 negatively regulates expression of TNFSF6/FASL and IL2 production in T-cells. It also binds to the TEA promoter and may participate in the regulation of DNA accessibility in the TCR-Jalpha locus.

SUBCELLULAR LOCATION: Nucleus Probable.

TISSUE SPECIFICTY: Isoform 1 is widely expressed with highest levels in muscle, kidney and liver. Isoform 2 is expressed primarily in immature thymocytes. Neither isoform is expressed in spleen or bone marrow.

SEQUENCE SIMILARITIES: Belongs to the nuclear hormone receptor family. NR1 subfamily.

Contains 1 nuclear receptor DNA-binding domain.
Molecular Weight~52 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in EL4 cell lysate.

Western Blot Analysis: 2 µg/mL of this antibody detected Retinoid-Related Orphan Receptor γ T (RORγT) in 10 µg of EL4 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
04-959 04053252641503

Documentation

Anti-Retinoid-Related Orphan Receptor γ T Antibody, clone RORg2 SDS

Title

Safety Data Sheet (SDS) 

Anti-Retinoid-Related Orphan Receptor γ T Antibody, clone RORg2 Certificates of Analysis

TitleLot Number
Anti-Retinoid-Related Orphan Receptor T, clone RORg2 - 1951088 1951088
Anti-Retinoid-Related Orphan Receptor T, clone RORg2 - 2224484 2224484
Anti-Retinoid-Related Orphan Receptor T, clone RORg2 - 2358865 2358865
Anti-Retinoid-Related Orphan Receptor T, clone RORg2 - NG1898232 NG1898232
Anti-Retinoid-Related Orphan Receptor T, clone RORg2 - NRG1776165 NRG1776165
Anti-Retinoid-Related Orphan Receptor #947; T, clone RORg2 - 2039822 2039822
Anti-Retinoid-Related Orphan Receptor γ T, clone RORg2 - 2461553 2461553
Anti-Retinoid-Related Orphan Receptor γ T, clone RORg2 - 3574546 3574546
Anti-Retinoid-Related Orphan Receptor γ T, clone RORg2 - 4051802 4051802
Anti-Retinoid-Related Orphan Receptor γ T, clone RORg2 -2721659 2721659
Show more