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MABN2613-100UG Anti-RTN/NOGO-A Antibody, clone NG1

Anti-RTN/NOGO-A, clone NG1, Cat. No. MABN2613, is an Armenian hamster monoclonal antibody that detects Reticulon-4 and is tested for use in Immunohistochemistry.

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MABN2613-100UG
100 μg  
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Overview

Replacement Information
Description
Catalogue NumberMABN2613-100UG
DescriptionAnti-RTN/NOGO-A Antibody, clone NG1
Alternate Names
  • Reticulon-4
  • Neurite outgrowth inhibitor
  • Nogo protein
Background InformationReticulon-4 (UniProt: Q99P72; also known as Neurite outgrowth inhibitor, Nogo protein) is encoded by the Rtn4 (also known as Kiaa0886, Nogo) gene (Gene ID: 68585) in murine species. NOGO proteins are required for the formation and stabilization of endoplasmic reticulum (ER) tubules. They are also involved in nuclear envelope expansion, nuclear pore complex formation and proper localization of inner nuclear membrane proteins. Three isoforms of NOGO have been described that share a common C-terminal domain but differ in their N-terminal sequence. NOGO-A is the longest isoform and is considered as a developmental neurite growth factor. It acts as a negative regulator of axon-axon adhesion and growth, but also acts as a facilitator of neurite branching. In the adult central nervous system (CNS), it is involved in down-regulation of growth, stabilization of wiring, and restriction of plasticity. It can also serve as a negative regulator of CNS angiogenesis by blocking spreading, migration, and sprouting of primary brain microvascular endothelial cells. Isoform B mainly functions in the endothelial cells and vascular smooth muscle cells. It promotes the migration of endothelial cells but inhibits the migration of vascular smooth muscle cells. Isoform C regulates cardiomyocyte apoptosis under hypoxic conditions. Clone NG1 recognizes NOGA-A but does not react with other isoforms. It is shown to strongly bind olfactory bulb axons in the lateral olfactory tract as well as some other growing axons in nervous system. This clone has been use to show that knockdown of NOGO-A reduces the number of axonal collateral branches in olfactory bulb. (Ref.: Iketani, M., et al. (2016). Sci. Rep. 6; 39586; Petrinovic, MM., et al. (2010). Development. 137(15); 2539-2550; Tozaki, H., et al. (2002). Mol. Brain Res. 104(2); 111-119).
References
Product Information
FormatPurified
PresentationPurified armenian hamster monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-RTN/NOGO-A, clone NG1, Cat. No. MABN2613, is an Armenian hamster monoclonal antibody that detects Reticulon-4 and is tested for use in Immunohistochemistry.
Key Applications
  • Immunohistochemistry
Application NotesTested Applications

Immunohistochemistry Application: A representative lot detected RTN/NOGO-A in Immunohistochemistry applications (Iketani, M., et al. (2016). Sci Rep. 6:39586; Tozaki, H., et al. (2002). Brain Res Mol Brain Res. 104(2):111-9).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenLateral olfactory tract of E14.5 mouse embryos fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS), washed and suspended in PBS.
EpitopeUnknown
CloneNG1
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostArmenian Hamster
SpecificityClone NG1 is an Armenian Hamster monoclonal antibody that detects murine Reticulon-4 (RTN4).
IsotypeIgG
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Rtn4
Purification MethodProtein G purified
UniProt Number
Molecular Weight126.61 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in E14 mouse embryo frozen tissue sections.

Immunohistochemistry Applications: A 1:200 dilution of this antibody detected RTN/NOGO-A in E14 mouse embryo frozen tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN2613-100UG 04065266582307