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MAB5328 Anti-RAGE Antibody, clone DD/A11 or A11

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MAB5328
100 µg  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
B, H, MELISA, ICC, IHC, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB5328
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-RAGE Antibody, clone DD/A11 or A11
Alternate Names
  • Receptor for Advanced Glycosylation End Products.
Background InformationRAGE (Receptor for Advanced Glycosylation End Products) is a 35 kDa cell surface receptor that binds molecules modified by advanced glycation end products (AGEs), an irreversible non-enzymatic reaction occuring from the interaction of proteins and lipids with glucose. The increased expression of RAGE is associated with many disorders, such as diabetic vasculopathy, neuropathy, retinopathy and neuropathy, Alzheimer’s disease and immune/inflammatory reactions of the vessel walls. AGE/RAGE accumulates at sites of vascular disease in diabetes, but blockade of RAGE suppresses development of atherosclerosis and vascular disorders. RAGE binds to amyloid beta, which is overproduced in neurons and vessels in Alzheimer's disease, and this interaction results in oxidative stress leading to neuronal degeneration.
References
Product Information
FormatPurified
HS Code3002 15 90
Control
  • Lung tissue, mouse brain lysate.
PresentationPurified mouse monoclonal IgG2a in buffer containing 0.02 M phosphate buffer, 0.25 M NaCl, and 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-RAGE Antibody, clone DD/A11 or A11 is an antibody against RAGE for use in ELISA, IC, IH & WB.
Key Applications
  • ELISA
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blotting
Application NotesImmunohistochemistry:
A previous lot of this antibody was shown to be reactive on paraffin embedded tissue sections.

Immunocytochemistry:
A previous lot was shown to work on cells fixed with ice cold acetone using a Cy3 conjugated secondary antibody.

ELISA:
A previous lot was shown to work on direct ELISA.

Immunoblotting:
Recognizes a band of ~48kDa in bovine lung extract.

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenRecombinant truncated extracellular part of RAGE produced in E. coli.
EpitopeExtracellular
CloneDD/A11 or mAbA11
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityRAGE (Receptor for Advanced Glycosylated End Products). The antibody recognizes both natural and recombinant RAGE.
IsotypeIgG2a
Species Reactivity
  • Bovine
  • Human
  • Mouse
Species Reactivity NoteMouse. Expected to react with rat based on sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • RAGE
  • RAGE-1
  • MOK
  • RAGE1
  • EC 2.7.11.22
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q15109 # Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Receptor for amyloid beta peptide.
SIZE: 404 amino acids; 42803 Da
SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted.
TISSUE SPECIFICITY: Endothelial cells.
PTM: Autophosphorylated (By similarity).
SIMILARITY: SwissProt: Q15109 # Contains 2 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Molecular Weight48 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by Western Blot on Mouse Brain lysates.

Western Blot:
1:500 dilution of this lot detected RAGE on 10 μg of Mouse Brain lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB5328 04053252611162

Documentation

Anti-RAGE Antibody, clone DD/A11 or A11 SDS

Title

Safety Data Sheet (SDS) 

Anti-RAGE Antibody, clone DD/A11 or A11 Certificates of Analysis

TitleLot Number
Anti-RAGE, clone DD/A11 or mAbA11 - 2118037 2118037
Anti-RAGE, clone DD/A11 or mAbA11 - 2428726 2428726
Anti-RAGE, clone DD/A11 or mAbA11 - 2443160 2443160
Anti-RAGE, clone DD/A11 or mAbA11 - 1983683 1983683
Anti-RAGE, clone DD/A11 or mAbA11 - 2041579 2041579
Anti-RAGE, clone DD/A11 or mAbA11 - 2300465 2300465
Anti-RAGE, clone DD/A11 or mAbA11 - 2942483 2942483
Anti-RAGE, clone DD/A11 or mAbA11 - 3225898 3225898
Anti-RAGE, clone DD/A11 or mAbA11 - 3283673 3283673
Anti-RAGE, clone DD/A11 or mAbA11 - 3448963 3448963

References

Reference overviewApplicationSpeciesPub Med ID
Receptor for Advanced Glycation End-Products Signaling Interferes with the Vascular Smooth Muscle Cell Contractile Phenotype and Function.
Simard, E; Söllradl, T; Maltais, JS; Boucher, J; D'Orléans-Juste, P; Grandbois, M
PloS one  10  e0128881  2015

Show Abstract
26248341 26248341
Statins stimulate the production of a soluble form of the receptor for advanced glycation end products.
Quade-Lyssy, P; Kanarek, AM; Baiersdörfer, M; Postina, R; Kojro, E
Journal of lipid research  54  3052-61  2013

Show Abstract
23966666 23966666
AGEs-RAGE mediated up-regulation of connexin43 in activated human microglial CHME-5 cells.
Shamim B Shaikh,Benedict Uy,Amali Perera,Louise F B Nicholson
Neurochemistry international  60  2012

Show Abstract
22417726 22417726
Induction of RAGE shedding by activation of G protein-coupled receptors.
Metz, VV; Kojro, E; Rat, D; Postina, R
PloS one  7  e41823  2012

Show Abstract
22860017 22860017
Fisetin lowers methylglyoxal dependent protein glycation and limits the complications of diabetes.
Maher, P; Dargusch, R; Ehren, JL; Okada, S; Sharma, K; Schubert, D
PloS one  6  e21226  2011

Show Abstract
Western BlottingMouse21738623 21738623
Gradual increase of high mobility group protein b1 in the lungs after the onset of acute exacerbation of idiopathic pulmonary fibrosis.
Ebina, M; Taniguchi, H; Miyasho, T; Yamada, S; Shibata, N; Ohta, H; Hisata, S; Ohkouchi, S; Tamada, T; Nishimura, H; Ishizaka, A; Maruyama, I; Okada, Y; Takashi, K; Nukiwa, T
Pulmonary medicine  2011  916486  2011

Show Abstract Full Text Article
21637372 21637372
Alternatively spliced RAGEv1 inhibits tumorigenesis through suppression of JNK signaling.
Kalea, Anastasia Z, et al.
Cancer Res., 70: 5628-38 (2010)  2010

Show Abstract
20570900 20570900
HMGB1 as an autocrine stimulus in human T98G glioblastoma cells: role in cell growth and migration.
Rosaria Bassi, Paola Giussani, Viviana Anelli, Thomas Colleoni, Marco Pedrazzi, Mauro Patrone, Paola Viani, Bianca Sparatore, Edon Melloni, Laura Riboni, Rosaria Bassi, Paola Giussani, Viviana Anelli, Thomas Colleoni, Marco Pedrazzi, Mauro Patrone, Paola Viani, Bianca Sparatore, Edon Melloni, Laura Riboni
Journal of neuro-oncology  87  23-33  2008

Show Abstract
17975708 17975708
A soluble form of the receptor for advanced glycation endproducts (RAGE) is produced by proteolytic cleavage of the membrane-bound form by the sheddase a disintegrin and metalloprotease 10 (ADAM10).
Raucci, A; Cugusi, S; Antonelli, A; Barabino, SM; Monti, L; Bierhaus, A; Reiss, K; Saftig, P; Bianchi, ME
FASEB journal : official publication of the Federation of American Societies for Experimental Biology  22  3716-27  2008

Show Abstract
18603587 18603587
Neural-activity-dependent release of S100B from astrocytes enhances kainate-induced gamma oscillations in vivo.
Sakatani, S; Seto-Ohshima, A; Shinohara, Y; Yamamoto, Y; Yamamoto, H; Itohara, S; Hirase, H
The Journal of neuroscience : the official journal of the Society for Neuroscience  28  10928-36  2008

Show Abstract
18945900 18945900

Brochure

Title
Pathways and Biomarkers of Oxidative Stress

Technical Info

Title
White Paper- Modern Methods in Oxidative Stress Research