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MABS1758 Anti-RAD9A Antibody, clone 5H8.1

Detect RAD9A using this mouse monoclonal Anti-RAD9A Antibody, clone 5H8.1, Cat. No. MABS1758, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-RAD9A Antibody, clone 5H8.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: RAD9A, RAD9 UniProt Number: Q99638
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MABS1758
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIH(P), WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1758
DescriptionAnti-RAD9A Antibody, clone 5H8.1
Alternate Names
  • Cell cycle checkpoint control protein RAD9A
  • DNA repair exonuclease rad9 homolog A
  • hRAD9
  • Rad9-like protein
Background InformationCell cycle checkpoint control protein RAD9A (EC 3.1.11.2; UniProt Q99638; also known as DNA repair exonuclease rad9 homolog A, hRAD9, Rad9-like protein) is encoded by the RAD9A (also known as RAD9A) gene (Gene ID 5883) in human. RAD9A is a component of the trimeric PCNA-like 9-1-1 complex (Rad9A, Rad1, and Hus1) and plays an important role in maintaining genomic integrity. RAD9A participates in both ATR and ATM signaling pathways-mediated cell cycle checkpoints in mitosis. Evidences indicate that RAD9A participates in DNA damage repair by interacting with proteins involved in base excision repair, mismatch repair and homologous recombination repair. RAD9A is shown to colocalize with γH2AX following DNA damage induction in an ATM-independent manner, and RAD9A inactivation delays the appearance of ionizing radiation-induced γH2AX foci. Rad9a-knockout causes embryonic lethality in mice due to increased apoptotic activity and reduced cellular proliferation, while mice heterozygous for Rad9a are prone to spontaneous and radiation-induced cataractogenesis. In addition, conditional knockout in keratinocytes causes enhanced susceptibility to carcinogen-induced skin tumors, while conditional Rad9a deletion in undifferentiated spermatogonia results in drastic effects on meiosis.
References
Product Information
FormatPurified
PresentationPurified mouse IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect RAD9A using this mouse monoclonal Anti-RAD9A Antibody, clone 5H8.1, Cat. No. MABS1758, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected nuclear RAD9A immunoreactivity in human prostate cancer, pancreas, and small intestine tissue sections.
Biological Information
ImmunogenGST-tagged recombinant human RAD9A ABL1-interacting domain fragment.
Clone5H8.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 5H8.1 targeted epitope lies within RAD9A ABL1-interacting domain.
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • RAD9A
  • RAD9
Purification MethodProtein G purified
UniProt Number
Molecular Weight~42 kDa observed. 42.55 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human skeletal muscle tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected RAD9A in 10 µg of human skeletal muscle tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS1758 04054839114526