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DP10L Anti-Polyoma Virus T Antigen (Ab-4) Mouse mAb (F4)

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DP10L
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
Polyoma Infected CellsMMonoclonal Antibody
Description
Overview

This product has been discontinued.



Recognize polyoma virus large, middle, and small T antigens.
Catalogue NumberDP10L
Brand Family Calbiochem®
References
ReferencesBolen, J.B., et al. 1984. Cell 38, 767.
Courtneidge, S.A. and Smith A.E. 1984. EMBO J. 3, 585.
Courtneidge, S.A. and Smith, A.E. 1983. Nature 303, 435.
Eckhart, W. 1981. Adv. Cancer Res. 35, 1.
Ito, Y., et al. 1980. J. Virol. 35, 219.
Ito, Y. and Spurr, N. 1980. Cold Spring Harbor Symp. Quant. Biol. 44, 149.
Turler, H. 1980. Mol. Cell. Biochem. 32, 63.
Smith, A.E., et al. 1979. Cell 18, 915.
Eckhart, W., et al. 1979. Cell 18, 925.
Schaffhausen, B.S. and Benjamin, T.L. 1979. Cell 18, 935.
Product Information
FormLyophilized
FormulationLyophilized from a volatile buffer, 100 µg BSA.
Positive controlPolyoma infected 3T6 cells
PreservativeNone
Quality LevelMQ100
Applications
Application ReferencesOriginal Clone Pallas, D., et al. 1986. J. Virol. 60, 1075.
Key Applications Immunofluorescence
Immunoprecipitation
Not Western Blot
Application NotesImmunofluorescence (1-5 µg/ml)
Immunoprecipitation (1-2 µg/ml)
Immunoblotting (not recommended)
Application CommentsFor immunoprecipitation, label by autophosphorylation. Antibody should be titrated for optimal results in individual sample types.
Biological Information
ImmunogenPolyoma virus T antigen
CloneF4
HostMouse
IsotypeIgG₁
Species Reactivity
  • Polyoma Infected Cells
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsResuspend the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline, pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
DP10L 0

Documentation

Anti-Polyoma Virus T Antigen (Ab-4) Mouse mAb (F4) SDS

Title

Safety Data Sheet (SDS) 

Anti-Polyoma Virus T Antigen (Ab-4) Mouse mAb (F4) Certificates of Analysis

TitleLot Number
DP10L

References

Reference overview
Bolen, J.B., et al. 1984. Cell 38, 767.
Courtneidge, S.A. and Smith A.E. 1984. EMBO J. 3, 585.
Courtneidge, S.A. and Smith, A.E. 1983. Nature 303, 435.
Eckhart, W. 1981. Adv. Cancer Res. 35, 1.
Ito, Y., et al. 1980. J. Virol. 35, 219.
Ito, Y. and Spurr, N. 1980. Cold Spring Harbor Symp. Quant. Biol. 44, 149.
Turler, H. 1980. Mol. Cell. Biochem. 32, 63.
Smith, A.E., et al. 1979. Cell 18, 915.
Eckhart, W., et al. 1979. Cell 18, 925.
Schaffhausen, B.S. and Benjamin, T.L. 1979. Cell 18, 935.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision22-August-2007 RFH
ApplicationImmunofluorescence (1-5 µg/ml)
Immunoprecipitation (1-2 µg/ml)
Immunoblotting (not recommended)
DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~100 kDa large T antigen, the ~55 kDa middle T antigen, and the ~22 kDa small T antigen of polyoma virus.
BackgroundPolyoma virus T-Ag (Ab-4) is a broadly reactive monoclonal antibody which recognizes the large, middle and small T antigens in polyoma virus transformed or infected cells. Polyoma virus is a small DNA tumor virus capable of lytically infecting mouse cells in culture and of transforming other rodent cells (rat and hamster fibroblasts, predominantly). The "early region" of polyoma virus DNA codes for three major polypeptides. These proteins have been designated large T antigen (~100 kDa), middle T antigen (~55 kDa) and small T antigen (~22 kDa). These large, middle, and small T antigens share N-terminal amino acid sequences. However, because the middle T antigen mRNA is spliced into a different reading frame from the large T antigen at the C-terminal portion of the two proteins, they exhibit distinct differences in cellular location and function.
HostMouse
ImmunogenPolyoma virus T antigen
CloneF4
IsotypeIgG₁
Speciespolyoma infected cells
Positive controlPolyoma infected 3T6 cells
FormLyophilized
FormulationLyophilized from a volatile buffer, 100 µg BSA.
PreservativeNone
CommentsFor immunoprecipitation, label by autophosphorylation. Antibody should be titrated for optimal results in individual sample types.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsResuspend the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline, pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles of solutions.
Toxicity Standard Handling
ReferencesBolen, J.B., et al. 1984. Cell 38, 767.
Courtneidge, S.A. and Smith A.E. 1984. EMBO J. 3, 585.
Courtneidge, S.A. and Smith, A.E. 1983. Nature 303, 435.
Eckhart, W. 1981. Adv. Cancer Res. 35, 1.
Ito, Y., et al. 1980. J. Virol. 35, 219.
Ito, Y. and Spurr, N. 1980. Cold Spring Harbor Symp. Quant. Biol. 44, 149.
Turler, H. 1980. Mol. Cell. Biochem. 32, 63.
Smith, A.E., et al. 1979. Cell 18, 915.
Eckhart, W., et al. 1979. Cell 18, 925.
Schaffhausen, B.S. and Benjamin, T.L. 1979. Cell 18, 935.
Application referencesOriginal Clone Pallas, D., et al. 1986. J. Virol. 60, 1075.