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DR1037 Anti-Plk1 Mouse mAb (35-206)

Anti-Plk1 Mouse mAb (35-206) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Polo-Like Kinase

DR1037
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~62 kDa Plk1 protein in HeLa cells.
Catalogue NumberDR1037
Brand Family Calbiochem®
SynonymsAnti-Polo-Like Kinase
Application Data
Detection of human Plk1 by immunoblotting. Sample: Whole cell lysate (50 µg) from HeLa cells treated with nocodazole (100 ng/ml). Primary antibody: Anti-Plk1 Mouse mAb (35-206) (Cat. No. DR1037) (1 µg/ml). Detection: chemiluminescence.

Detection of human Plk1 by immunoprecipitation and immunoblotting. Sample: Whole cell lysate (446 µg) from HeLa cells treated with nocodazole (100 ng/ml). Antibody for IP: Anti-Plk1 Mouse mAb (35-206) (Cat. No. DR1037) (5 µg). Conditions for immunoblotting: same as above.
References
ReferencesTakai, N., et al. 2005. Oncogene 24, 287.
Toyoshima-Morimoto, F., et al. 2002. EMBO Rep. 4, 341.
Smits, V.A., et al. 2000. Nat. Cell Biol. 2, 672.
Glover, D.M., et al. 1998. Genes Devel. 12, 3777.
Product Information
FormLiquid
FormulationIn PBS.
Positive controlNocodazole-treated HeLa cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesImmunoblotting (1 µg/ml)
Immunoprecipitation (10 µg/ml)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenfull-length, recombinant, human Plk1
ImmunogenHuman
Clone35-206
HostMouse
IsotypeIgG2b
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Irritant
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
DR1037 0

Documentation

Anti-Plk1 Mouse mAb (35-206) SDS

Title

Safety Data Sheet (SDS) 

Anti-Plk1 Mouse mAb (35-206) Certificates of Analysis

TitleLot Number
DR1037

References

Reference overview
Takai, N., et al. 2005. Oncogene 24, 287.
Toyoshima-Morimoto, F., et al. 2002. EMBO Rep. 4, 341.
Smits, V.A., et al. 2000. Nat. Cell Biol. 2, 672.
Glover, D.M., et al. 1998. Genes Devel. 12, 3777.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision23-August-2007 RFH
SynonymsAnti-Polo-Like Kinase
ApplicationImmunoblotting (1 µg/ml)
Immunoprecipitation (10 µg/ml)
Application Data
Detection of human Plk1 by immunoblotting. Sample: Whole cell lysate (50 µg) from HeLa cells treated with nocodazole (100 ng/ml). Primary antibody: Anti-Plk1 Mouse mAb (35-206) (Cat. No. DR1037) (1 µg/ml). Detection: chemiluminescence.

Detection of human Plk1 by immunoprecipitation and immunoblotting. Sample: Whole cell lysate (446 µg) from HeLa cells treated with nocodazole (100 ng/ml). Antibody for IP: Anti-Plk1 Mouse mAb (35-206) (Cat. No. DR1037) (5 µg). Conditions for immunoblotting: same as above.
DescriptionProtein G purified mouse monoclonal antibody. Recognizes the ~62 kDa Plk1 protein.
BackgroundPlk1 (ACC# NP_005021) is an evolutionarily conserved member of the polo-like serine/threonine kinase family that plays a critical role in spindle formation, chromosome segregation, regulation of the anaphase-promoting complex (APC), and execution of cytokinesis. Predominantly cytoplasmic during interphase, Plk1 is associated with condensed chromatin during mitosis when its kinase activity peaks. Plk1 regulates mitotic events through phosphorylation of its substrates Cdc25, the activator of Cdc2, and Cdc27, a component of the APC. Phosphorylation of Cdc25C by Plk1 is thought to lead to nuclear accumulation of Cdc25 and activation of Cdc2. Phosphorylation of Cdc27 leads to destruction of the APC. Ectopic expression of Plk1 in NIH3T3 leads to transformation. Over-expression of Plk1 is positively correlated with aggressiveness and prognosis of many cancers and inhibition of Plk1 activity results in growth arrest and apoptosis in cancer cells. Plk1 has been shown to be elevated in numerous cancers including lung, breast, ovarian, melanoma, colorectal, and gliomas.
HostMouse
Immunogen speciesHuman
Immunogenfull-length, recombinant, human Plk1
Clone35-206
IsotypeIgG2b
Specieshuman, mouse, rat
Positive controlNocodazole-treated HeLa cells
FormLiquid
FormulationIn PBS.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Irritant
ReferencesTakai, N., et al. 2005. Oncogene 24, 287.
Toyoshima-Morimoto, F., et al. 2002. EMBO Rep. 4, 341.
Smits, V.A., et al. 2000. Nat. Cell Biol. 2, 672.
Glover, D.M., et al. 1998. Genes Devel. 12, 3777.