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ABT59 Anti-Pericentrin Antibody

This Anti-Pericentrin Antibody is validated for use in WB, IC for the detection of Pericentrin.

Anti-Pericentrin Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeleton Gene Symbol: PCNT, PCNT2 UniProt Number: O95613
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ABT59
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABT59
DescriptionAnti-Pericentrin Antibody
Alternate Names
  • Pericentrin
  • Kendrin
  • Pericentrin-B
Background InformationPericentrin binds to calmodulin and is expressed in the centrosome that serves as a multifunctional scaffold for anchoring proteins and protein complexes. Evidence suggests that pericentrin plays a role in microtubule nucleation and spindle organization. Recent studies show pericentrin plays a role in Majewski/micorcephalic osteodysplastic primordial dwarfism type II (MOPDII) and Seckel Syndrome. Recent use in network modeling indicates a direct link between breast cancer susceptibility to centrosome defects. Pericentrin is a potential marker for centrosome defects in cancer and cancer progression, as centrosomal abonormalities in solid tumors are accompanied by increased pericentrin levels and defects in pericentrin organization.
References
Product Information
FormatAffinity Purified
Control
  • HeLa cell lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Pericentrin Antibody is validated for use in WB, IC for the detection of Pericentrin.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Pericentrin in HeLa cells.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human Pericentrin.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
Species Reactivity
  • Human
Species Reactivity NoteDemonstrated to react with Human.
Other homologies: Mouse (89% sequence homology). No homology to Rat was found.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene binds to calmodulin and is expressed in the centrosome. It is an integral component of the pericentriolar material (PCM). The protein contains a series of coiled-coil domains and a highly conserved PCM targeting motif called the PACT domain near its C-terminus. The protein interacts with the microtubule nucleation component gamma-tubulin and is likely important to normal functioning of the centrosomes, cytoskeleton, and cell-cycle progression. Mutations in this gene cause Seckel syndrome-4 and microcephalic osteodysplastic primordial dwarfism type II. [provided by RefSeq].
Gene Symbol
  • PCNT
  • PCNT2
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM).

SUBUNIT STRUCTURE: Interacts with CHD3. Interacts with CHD4; the interaction regulates centrosome integrity (By similarity). Interacts with DISC1 and PCM1. Binds calmodulin. Interacts with CDK5RAP2; the interation is leading to centrosomal localization of PCNT and CDK5RAP2.

SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton › centrosome. Note: Centrosomal at all stages of the cell cycle. Remains associated with centrosomes following microtubule depolymerization. Colocalized with DISC1 at the centrosome.

TISSUE SPECIFICITY: Expressed in all tissues tested, including placenta, liver, kidney and thymus.

DOMAIN: Composed of a coiled-coil central region flanked by non-helical N- and C-terminals.

INVOLVEMENT IN DISEASE: Defects in PCNT are the cause of microcephalic osteodysplastic primordial dwarfism type 2 (MOPD2) [MIM:210720]; also known as osteodysplastic primordial dwarfism type 2. Adults with this rare inherited condition have an average height of 100 centimeters and a brain size comparable to that of a 3-month-old baby, but are of near-normal intelligence.

SEQUENCE CAUTION: The sequence AAD10838.1 differs from that shown. Reason: Frameshift at position 3320.

The sequence BAA23698.3 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

The sequence BAC04252.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
Molecular Weight~250 kDa observed.
Uniprot describes 2 isoforms produced by alternative splicing with molecular weights at 378 & 355 kDa. However in endogenous lysates, Pericentrin is observed at ~250 kDa & ~150 kDa for western blots (Wen, Y., et al. (2008). J Biol Chem. 283(5):2793-2803).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Pericentrin in 10 µg of HeLa cell lysate. Western Blot was performed without Tween.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABT59 04053252420634

Documentation

Anti-Pericentrin Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-Pericentrin Antibody Certificates of Analysis

TitleLot Number
Anti-Pericentrin - 2345062 2345062
Anti-Pericentrin - 2385981 2385981
Anti-Pericentrin - 2271194 2271194
Anti-Pericentrin - 2293545 2293545
Anti-Pericentrin - 3217087 3217087
Anti-Pericentrin - 3460371 3460371
Anti-Pericentrin - 3527767 3527767
Anti-Pericentrin - 3723484 3723484
Anti-Pericentrin - 4153071 4153071
Anti-Pericentrin - NRG1916844 NRG1916844
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