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ABE1947-25UG Anti-PRDM9

Anti-PRDM9, Cat. No. ABE1947, is a highly specific rabbit polyclonal antibody that targets Histone-lysine N-methyltransferase PRDM9 in human and murine cells and has been tested for use in Western Blotting.

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ABE1947-25UG
25 μg  
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Overview

Replacement Information
Description
Catalogue NumberABE1947-25UG
DescriptionAnti-PRDM9
Alternate Names
  • Histone-lysine N-methyltransferase PRDM9
  • EC: 2.1.1.43
  • PR domain zinc finger protein 9
  • PR domain-containing protein 9
Background InformationHistone-lysine N-methyltransferase PRDM9 (UniProt: Q9NQV7; EC: 2.1.1.43, PR domain zinc finger protein 9, PR domain-containing protein 9, PRDM9) is encoded by PRDM9 (also known as PFM6) gene (Gene ID: 56979) in human. PRDM9 is a zinc-finger protein that catalyzes histone H3 Lysine 4 trimethylation (H3K4me3), but lacks capacity to mono- and dimethylate Lysine 4 of histone H3. H3 Lysine -4 methylation plays a vital role in the transcriptional activation of genes during early meiotic prophase. PRDM9 contains multiple domains, including a Kruppel-associated box (KRAB) domain (aa 23-86), an SSX repression domain (SSXRD), a PRD1-BF1 and RIZ homologous region, a subclass of SET (PR/SET) domain (aa 244-358), and a tandem array of C2H2 zinc fingers. The zinc finger array recognizes a short sequence motif, leading to local H3K4me3, and meiotic recombination hotspot activity. PRDM9 is reported to be highly polymorphic, both within and between mammalian species. It is shown that most of the naturally occurring sequence polymorphisms in PRDM9 change the identity of the amino acids contacting DNA and/or the number and arrangement of individual fingers in the DNA-binding zinc-finger domains. This allows PRDM9 variants to target a large number of DNA sequences, thereby expanding the distribution of recombination sites. (Ref.: Baker, CL et al. (2015). PLoS Genet 11(9): e1005512).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-PRDM9, Cat. No. ABE1947, is a highly specific rabbit polyclonal antibody that targets Histone-lysine N-methyltransferase PRDM9 in human and murine cells and has been tested for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A representaive lot detected PRDM9 in Wild type, but not in KO mouse testis tissue lysate ( Courtesy  of Guccione Lab @ A*STAR( Institute of Molecular Cell Biology, IMCB) ).
Biological Information
ImmunogenGST-tagged recombinant fragment corresponding to 191 amino acids from the N-terminal half of human Histone-lysine N-methyltransferase PRDM9.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Histone-lysine N-methyltransferase PRDM9 in human and mouse cells. It targets an epitope within 191 amino acids from the N-terminal half.
IsotypeIgG
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteHuman, Mouse.
Antibody TypePolyclonal Antibody
Gene Symbol
  • PRDM9
  • PFM6
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~90 kda observed; 103.37 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse testis tissue lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected PRDM9 in 10 µg of mouse testis tissue lysate .
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE1947-25UG 04054839526893