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MABE1990-100UG Anti-PLZF/ZBTB16 Antibody, clone Mags-21F7

Anti-PLZF/ZBTB16, clone Mags-21F7, Cat. No. MABE1990, is an Armenian hamster monoclonal antibody that detects PLZF/ZBTB16 and is tested for use in Flow Cytometry and Western Blotting.

MABE1990-100UG
100 μg  
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Overview

Replacement Information
Description
Catalogue NumberMABE1990-100UG
DescriptionAnti-PLZF/ZBTB16 Antibody, clone Mags-21F7
Alternate Names
  • Zinc finger and BTB domain-containing protein 16
  • Promyelocytic leukemia zinc finger protein
  • Zinc finger protein 145
  • Zinc finger protein PLZF
Background InformationZinc finger and BTB domain-containing protein 16 (UniProt: Q05516; also known as Promyelocytic leukemia zinc finger protein, Zinc finger protein 145, Zinc finger protein PLZF) is encoded by the ZBTB16 (also known as PLZF, ZNF145) gene (Gene ID: 7704) in human. PLZF is a member of the krueppel C2H2-type zinc-finger protein family with nine C2H2-tpe zinc-finger domains. It serves as a transcriptional repressor and this repression may be mediated through recruitment of histone deacetylases to target promoters. It affects diverse signaling pathways, including growth-regulatory and differentiation pathways and is a key regulator of myeloid development. Its expression is observed in early, but in differentiated hematopoietic cells. It is expressed in bone marrow, early myeloid cells, and peripheral blood mononuclear cells. At lower level it is also detected in the ovary, kidney, and lung tissue. PLZF is reported to be essential for development of the characteristic innate/effector functions of natural killer T (NKT) cells. It is highly expressed during the differentiation of NKT cells and is essential for the acquisition of effector/memory innate-like functions. PLZF-deficient NKT cells lose their characteristic memory phenotype, and their function is significantly impaired. These cells lose their unique ability to co-secrete IL-4 and IFN- upon ex vivo stimulation. (Ref.: Kolesnichenko, M., and Vogt, PK. (2011). Cell Cycle.10(5); 771-775; Kovalovsky, D., et al. (2010). J. Immunol. 184: 6746-6755; Melnick, AM., et al. (2000). Mol. Cell Biol. 20(6); 2075-2086).
References
Product Information
FormatPurified
PresentationPurified Armenian hamster monoclonal antibody IgG2-IgG3 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-PLZF/ZBTB16, clone Mags-21F7, Cat. No. MABE1990, is an Armenian hamster monoclonal antibody that detects PLZF/ZBTB16 and is tested for use in Flow Cytometry and Western Blotting.
Key Applications
  • Western Blotting
  • Flow Cytometry
Application NotesWestern Blotting Analysis: A representative lot detected PLZF/ZBTB16 in Western Blotting applications (Kovalovsky, D., et al. (2008). Nat Immunol. 9(9):1055-64).

Flow Cytometry Analysis: A representative lot detected PLZF/ZBTB16 in Flow Cytometry applications (Kovalovsky, D., et al. (2008). Nat Immunol.;9(9):1055-64; Kovalovsky, D., et al. (2010). J Immunol. 184(12):6746-55).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenA combination of peptides covering the amino, carboxyl, and hinge regions of human Zinc finger and BTB domain-containing protein 16 (PLZF/ZBTB16).
EpitopeUnknown
CloneMags-21F7
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostArmenian Hamster
SpecificityClone Mags-21F7 is an Armenian Hamster monoclonal antibody that detects Zinc finger and BTB domain-containing protein 16 (PLZF/ZBTB16).
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteMouse, Human.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ZBTB16
Purification MethodProtein G purified
UniProt Number
Molecular Weight~75 kDa observed; 74.27 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting with recombinant Human PLZF/ZBTB16 protein.

Western Blotting Analysis: A 1:500 dilution of this antibody detected full-length, FLAG-tagged recombinant Human PLZF/ZBTB16..
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE1990-100UG 04065270845306