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ST1203 Anti-PGC-1α Mouse mAb (1F3.9)

This Anti-PGC-1α Mouse mAb (1F3.9) is validated for use in Immunoblotting, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation for the detection of PGC-1α.

Anti-PGC-1α Mouse mAb (1F3.9) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody
Description
OverviewRecognizes the ~113 kDa PGC-1α protein in COS cells.
Catalogue NumberST1203
Brand Family Calbiochem®
Application Data
Detection of mouse PGC-1α by immunohistochemistry. Sample: Brown adipose tissue, fixed with acetone/ethanol, from a mouse held at room temperature (left and center panels) and from a mouse held at 4°C for 5 hr (right panel). Primary antibody: Anti-PGC-1α Mouse mAb (1F3.9) (Cat. No. ST1203) (1 µg/ml) (center and right panel only; left panel stained with secondary alone). Detection: DAB.

Detection of mouse PGC-1α by immunohistochemistry. Sample: Brown adipose tissue, fixed with acetone/ethanol, from a mouse held at room temperature (left and center panels) and from a mouse held at 4°C for 5 hr (right panel). Primary antibody: Anti-PGC-1α Mouse mAb (1F3.9) (Cat. No. ST1203) (1 µg/ml) (center and right panel only; left panel stained with secondary alone). Detection: fluorescence.

Detection of mouse PGC-1α by immunoblotting. Sample: Whole tissue extract from brown adipose tissue of mice held at room temperature (left 3 lanes) and mice held at 4°C for 5 hr (right three lanes). Primary antibody: Anti-PGC-1α Mouse mAb (1F3.9) (Cat. No. ST1203) (1 µg/ml). Detection: chemiluminescence.
References
ReferencesChang, J.S., et al. 2010. J. Biol. Chem. 285, 18039.
Zhang, Y. et al. 2009. J. Biol. Chem. 284, 32813.
Lai, L., et al. 2008. Genes Dev. 14, 1948.
Rodgers, J.T., et al. 2008. FEBS Lett. 582, 46.
Mazzucotelli, A., et al. 2007. Diabetes 10, 2467.
Nemoto, S., et al. 2005. J. Biol. Chem. 16, 16456.
Product Information
FormLiquid
FormulationIn 50 mM PBS
Positive controlBrown adipose tissue from mice exposed to cold
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunohistochemistry
Immunoprecipitation
Application NotesImmunoblotting (1 µg/ml)
Immunocytochemistry (5-10 µg/ml)
Immunohistochemistry (5-10 µg/ml)
Immunoprecipitation (1:100)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
ImmunogenA recombinant protein consisting of amino acids 1-120 of mouse PGC-1α
ImmunogenMouse
Clone1F3.9
HostMouse
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Regulatory Review
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ST1203 0

Documentation

Anti-PGC-1α Mouse mAb (1F3.9) SDS

Title

Safety Data Sheet (SDS) 

Anti-PGC-1α Mouse mAb (1F3.9) Certificates of Analysis

TitleLot Number
ST1203

References

Reference overview
Chang, J.S., et al. 2010. J. Biol. Chem. 285, 18039.
Zhang, Y. et al. 2009. J. Biol. Chem. 284, 32813.
Lai, L., et al. 2008. Genes Dev. 14, 1948.
Rodgers, J.T., et al. 2008. FEBS Lett. 582, 46.
Mazzucotelli, A., et al. 2007. Diabetes 10, 2467.
Nemoto, S., et al. 2005. J. Biol. Chem. 16, 16456.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision02-February-2011 RFH
ApplicationImmunoblotting (1 µg/ml)
Immunocytochemistry (5-10 µg/ml)
Immunohistochemistry (5-10 µg/ml)
Immunoprecipitation (1:100)
Application Data
Detection of mouse PGC-1α by immunohistochemistry. Sample: Brown adipose tissue, fixed with acetone/ethanol, from a mouse held at room temperature (left and center panels) and from a mouse held at 4°C for 5 hr (right panel). Primary antibody: Anti-PGC-1α Mouse mAb (1F3.9) (Cat. No. ST1203) (1 µg/ml) (center and right panel only; left panel stained with secondary alone). Detection: DAB.

Detection of mouse PGC-1α by immunohistochemistry. Sample: Brown adipose tissue, fixed with acetone/ethanol, from a mouse held at room temperature (left and center panels) and from a mouse held at 4°C for 5 hr (right panel). Primary antibody: Anti-PGC-1α Mouse mAb (1F3.9) (Cat. No. ST1203) (1 µg/ml) (center and right panel only; left panel stained with secondary alone). Detection: fluorescence.

Detection of mouse PGC-1α by immunoblotting. Sample: Whole tissue extract from brown adipose tissue of mice held at room temperature (left 3 lanes) and mice held at 4°C for 5 hr (right three lanes). Primary antibody: Anti-PGC-1α Mouse mAb (1F3.9) (Cat. No. ST1203) (1 µg/ml). Detection: chemiluminescence.
DescriptionProtein G purified mouse monoclonal antibody. Recognizes the ~113 kDa PGC-1α protein.
BackgroundPGC-1α is a co-activator of PPARα, PPARγ, and other transcription factors that regulates the transcriptonal program of adaptive thermogenesis in brown adipose tissue, hepatic/renal gluconeogenesis, and muscle fiber type switching. The full length protein is detected at 113 kDa and is induced in BAT by cold exposure, in liver and kidney by fasting, and in skeletal muscle by exercise. Alternative splicing of the full length gene produces a 270 aa N-terminal splice variant that migrates at ~38 kDa and is induced by the same physiological signals which induce expression of the full length protein.
HostMouse
Immunogen speciesMouse
ImmunogenA recombinant protein consisting of amino acids 1-120 of mouse PGC-1α
Clone1F3.9
IsotypeIgG
Specieshuman, mouse, rat
Positive controlBrown adipose tissue from mice exposed to cold
FormLiquid
FormulationIn 50 mM PBS
PreservativeNone
CommentsAntibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Regulatory Review
ReferencesChang, J.S., et al. 2010. J. Biol. Chem. 285, 18039.
Zhang, Y. et al. 2009. J. Biol. Chem. 284, 32813.
Lai, L., et al. 2008. Genes Dev. 14, 1948.
Rodgers, J.T., et al. 2008. FEBS Lett. 582, 46.
Mazzucotelli, A., et al. 2007. Diabetes 10, 2467.
Nemoto, S., et al. 2005. J. Biol. Chem. 16, 16456.