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ST1698 Anti-PDLIM7 Mouse mAb (2D6)

Anti-PDLIM7 Mouse mAb (2D6) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-PD2 and LIM Domain7

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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~45-50 kDa PDLIM7 protein in HeLa cells.
Catalogue NumberST1698
Brand Family Calbiochem®
SynonymsAnti-PD2 and LIM Domain7
Application Data
Detection of human PDLIM7 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-PDLIM7 Mouse mAb (2D6) (Cat. No. ST1698) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human PDLIM7 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-PDLIM7 Mouse mAb (2D6) (Cat. No. ST1698) (10 µg/ml). Detection: fluorescence.
References
ReferencesHerrick, S., et al. 2009. Mol. Cell Neurosci. 43, 188.
Product Information
Formliquid
FormulationIn PBS, pH 7.2.
Negative control293T cells
Positive controlHeLa cells
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Application NotesELISA (see coments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenFull-length, recombinant, human PDLIM7 (aa 1-458), expressed as a GST fusion protein
Clone2D6
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ST1698 0

Documentation

Anti-PDLIM7 Mouse mAb (2D6) SDS

Title

Safety Data Sheet (SDS) 

References

Reference overview
Herrick, S., et al. 2009. Mol. Cell Neurosci. 43, 188.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision10-November-2010 RFH
SynonymsAnti-PD2 and LIM Domain7
ApplicationELISA (see coments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Application Data
Detection of human PDLIM7 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-PDLIM7 Mouse mAb (2D6) (Cat. No. ST1698) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human PDLIM7 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-PDLIM7 Mouse mAb (2D6) (Cat. No. ST1698) (10 µg/ml). Detection: fluorescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~45-50 kDa PDLIM7 protein.
BackgroundThe protein encoded by this gene is representative of a family of proteins composed of conserved PDZ and LIM domains. LIM domains are proposed to function in protein-protein recognition in a variety of contexts including gene transcription and development and in cytoskeletal interaction. The LIM domains of this protein bind to protein kinases, whereas the PDZ domain binds to actin filaments. The gene product is involved in the assembly of an actin filament-associated complex essential for transmission of ret/ptc2 mitogenic signaling. The biological function is likely to be that of an adapter, with the PDZ domain localizing the LIM-binding proteins to actin filaments of both skeletal muscle and nonmuscle tissues. Alternative splicing of this gene results in multiple transcript variants.
HostMouse
ImmunogenFull-length, recombinant, human PDLIM7 (aa 1-458), expressed as a GST fusion protein
Clone2D6
IsotypeIgG₁
Specieshuman
Positive controlHeLa cells
Negative control293T cells
Formliquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review
ReferencesHerrick, S., et al. 2009. Mol. Cell Neurosci. 43, 188.